Acute hypoxia suppresses blood glycolysis in saker falcons(Falco cherrug)via NR3C1-mediated repression of HK1:Evidence from hematological and epigenomic profilingOACSCDCSTPCD
Ongoing climate change is driving high-altitude bird species to occupy even higher elevations,yet physiological and regulatory responses enabling these transitions remain poorly understood.This study investigated acute hypoxic responses in saker falcons(Falco cherrug)inhabiting the Qinghai-Xizang Plateau by exposing individuals to simulated altitudes of 5000-6000 m above sea level(a.s.l.),exceeding their typical elevation range(approximately 4300 m a.s.l.).GPS tracking data indicated that juvenile falcons maintained comparable activity levels across 4000-5000 m and 5000-6000 m a.s.l.ranges.However,pre-fledging individuals subjected to 6000 m hypoxia for three days exhibited marked increases in hemoglobin concentration and blood glucose.Transcriptomic profiling revealed significant suppression of glycolytic activity,notably characterized by reduced expression of hexokinase 1(HK1),a key enzymatic gene involved in the glycolytic pathway.ATAC-seq further identified enhanced chromatin accessibility within the HK1 locus under hypoxia,revealing two conserved cis-regulatory elements recognized by the transcription factor NR3C1 in the hypoxia-treated group.NR3C1 expression was negatively correlated with HK1.Notably,both elements were unique and evolutionarily conserved in avian taxa,suggesting a potential role in hypoxia resilience among highland birds.These findings provide mechanistic insights into the molecular and physiological strategies employed by sakers to tolerate acute hypoxic stress and inform conservation efforts for high-altitude bird species on the Qinghai-Xizang Plateau and other alpine ecosystems facing accelerating climate change.
Wei Wu;Xiao-Hang Zhang;Fei-Fei Du;Zhong-Ru Gu;Li Hu;Jun-Feng Chen;Zhen-Zhen Lin;Sheng-Kai Pan;Xiang-Jiang Zhan
State Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China Key Laboratory of Animal Ecology and Conservation Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China Cardiff University-Institute of Zoology Joint Laboratory for Biocomplexity Research,Chinese Academy of Sciences,Beijing 100101,China University of the Chinese Academy of Sciences,Beijing 100049,ChinaState Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China Key Laboratory of Animal Ecology and Conservation Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China Cardiff University-Institute of Zoology Joint Laboratory for Biocomplexity Research,Chinese Academy of Sciences,Beijing 100101,China University of the Chinese Academy of Sciences,Beijing 100049,ChinaState Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China Key Laboratory of Animal Ecology and Conservation Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China Cardiff University-Institute of Zoology Joint Laboratory for Biocomplexity Research,Chinese Academy of Sciences,Beijing 100101,ChinaState Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China Key Laboratory of Animal Ecology and Conservation Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China Cardiff University-Institute of Zoology Joint Laboratory for Biocomplexity Research,Chinese Academy of Sciences,Beijing 100101,ChinaState Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai,Shandong 264003,ChinaState Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China Key Laboratory of Animal Ecology and Conservation Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,ChinaState Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China Key Laboratory of Animal Ecology and Conservation Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China Cardiff University-Institute of Zoology Joint Laboratory for Biocomplexity Research,Chinese Academy of Sciences,Beijing 100101,ChinaState Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China Key Laboratory of Animal Ecology and Conservation Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China Cardiff University-Institute of Zoology Joint Laboratory for Biocomplexity Research,Chinese Academy of Sciences,Beijing 100101,China School of Nature Conservation,Beijing Forestry University,Beijing 100083,ChinaState Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China Key Laboratory of Animal Ecology and Conservation Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China Cardiff University-Institute of Zoology Joint Laboratory for Biocomplexity Research,Chinese Academy of Sciences,Beijing 100101,China Center for Excellence in Animal Evolution and Genetics,Chinese Academy of Sciences,Kunming,Yunnan 650201,China
生物科学
Hypoxic responseQinghai-Xizang PlateauSaker falconsGlycolysisClimate change
《Zoological Research》 2025 (5)
P.1165-1174,10
supported by the National Nature Science Foundation of China(32222012,32125005,32270455,32361133559)。
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