解毒生肌膏调控Ang/Tie2/VEGF信号通路促进糖尿病溃疡血管生成OA

Objective To explore the mechanism by which Jiedu Shengji Plaster promotes diabetic ulcer repair based on the signalling pathways of vascular endothelial growth factor(VEGF),angiopoietin(Ang),and tyrosine kinase(Tie2).Methods Forty-eight SD rats were randomly divided into the normal control(n=12)and model groups(n=36),induced via high-fat diet combined with intraperitoneal injection of streptozotocin to create full-thickness skin defects on the back,and the rat model of diabetes ulcer was established.The model rat were randomly divided into model group,detoxification group and sulfonamide group,12 per group.After disinfection,the normal and model groups received Vaseline cream,whereas the detoxification and sulfonamide groups were treated with Jiedu Shengji Plaster and sulfonamide silver,respectively,with daily dressing changes.Wound healing rates were calculated from images obtained at days 0,3,7,and 14.After 14 days,samples were collected for histological and molecular analyses.HE staining was used for tissue inflammation,granulation tissue,and vascular conditions.Collagen volume was detected by collagen staining.Immunohistochemistry was used to detect Ang1,Ang2,and Tie2 expressions in wounds,and Western Blot for VEGF,Ang1,Ang2,and Tie2 protein levels in wound tissues.Results After 7 and 14 days of wound treatment,compared with normal group,the wound healing rate decreased(P＜0.01),inflammation increased,collagen decreased(P＜0.01),VEGF expression decreased(P＜0.01),Ang1 and Tie2 protein expression around blood vessels and wound tissue protein expression level decreased(P＜0.01),and Ang2 protein expression around blood vessels and wound tissue expression level increased in the model group(P＜0.01).Compared with the model group,the detoxification group showed significantly increased wound healing rate(P＜0.01),decreased inflammation,increased neovascularization,increased collagen(P＜0.01),increased VEGF expression(P＜0.01),increased Ang1 and Tie2 protein expression around blood vessels and wound tissue protein expression level(P＜0.01),and decreased Ang2 expression in blood vessels and wound tissue expression level(P＜0.01).The sulfonamide group showed a reversal in wound healing rate,inflammatory infiltration,Ang1,Tie2,and Ang2,however.However,all were weaker than the detoxification group(P＜0.01,P＜0.05).Conclusion Jiedu Shengji Plaster promotes angiogenesis and accelerates the healing of diabetic ulcers simultaneously with early anti-inflammatory effects,and the mechanism may be related to Ang/Tie2/VEGF signalling pathway regulation.