MyH基因家族分析及在信阳水牛各部位肌肉中的表达差异研究OA

In order to further clarify the myosin heavy chain(MyH)family gene characteristics and gene functions,and to analyze the expression of its multiple isoforms in various parts of muscle tissues of Xinyang buffalo.In this study,we analyzed the motif and structural domains of the MyH family genes and mapped the evolutionary relationships by constructing a phylogenetic tree,grasped the functions of the gene family through the PPI network interactions map,GO enrichment analysis and KEGG enrichment analysis,and understood the muscle developmental characteristics of Xinyang buffaloes by detecting their expression in various parts of mus-cle tissues.The results showed that the MyH gene family contains three motifs and three conserved structural domains,and evolu-tionarily MyH1,MyH2 and MyH4 are relatively close to each other.Enrichment analysis revealed that the MyH gene family is main-ly enriched in the signaling pathways of actin cytoskeleton regulation,tight junctions and vascular smooth muscle contraction.Tis-sue expression profiling showed that MyH1,MyH2 and MyH4 were highly expressed in the foreleg and hind leg muscles,and MyH7 was the most highly expressed in the longissimus dorsi muscle.In summary,this study revealed the molecular characteristics,evolu-tionary relationships and functional enrichment pathways of the MyH gene family in Xinyang buffalo,and clarified its expression patterns in different muscle parts.