河南省200份小麦品种苗期茎基腐病抗性鉴定与全基因组关联分析OA

Fusarium crown rot(FCR)is a globally prevalent soil-borne fungal disease of wheat.In recent years,its incidence has steadily increased in the middle-lower Yangtze River and Huang-Huai wheat-growing regions of China.However,wheat cultivars with strong resistance remain scarce.As a result,the identification of resistant germplasm and the discovery of resistance genes have become key priorities in resistance breeding.In this study,the dominant FCR pathogen Fusarium pseudograminearum strain WZ-8A,isolated from the Huang-Huai wheat region,was used to evaluate seedling-stage resistance in 133 landrace cultivars and 67 modern cultivars from Henan province.Phenotypic data were integrated with wheat 660K SNP genotyping data to perform a genome-wide association study(GWAS).The results revealed that only eight cultivars—Tutoumai(Qixian),Zaoyangmai,Bai-quan 41,Kaimai 18,Zhoumai 24,04 Zhong 36,Yumai 35,and Zhongyu 3—exhibited moderate resistance.GWAS identified 30 significant loci,with two SNPs(AX-111055517 and AX-110584552)consistently detected across multiple models and environ-ments.Functional annotation and expression analysis of candidate regions suggested that TraesCS4B02G048500 may be a key resistance gene.This study identified eight moderately resistant wheat accessions at the seedling stage,and the significant loci and putative genes discovered provide valuable resources,insights,and a theoretical foundation for future FCR resistance breeding and related research.