发根农杆菌介导龙葵遗传转化体系优化OA

The aim of this study was to establish an Agrobacterium rhizogenes-mediated genetic transformation system for Solanum nigrum(black nightshade)with the goal of enhancing transformation efficiency and increasing its total flavonoid content.Based on this system,the recombinant plasmid PBI121-6flag-SnCHI was constructed,the hairy roots of SnCHI gene transformation were induced by Agrobacterium-mediated pathway,and the content of total flavonoids in the hairy roots of S.nigrum after SnCHI gene transformation was investigated.The results showed that the induction rate of hairy roots of S.nigrum could reach 88.49%under the conditions of OD600 value of 1.0,infection for 25 min and co-culture for 24 h.The qRT-PCR detection of transgenic SnCHI hairy roots showed that the gene expression level was 28.81 times that of the blank group.The total flavonoid content of SnCHI transgenic hairy roots was determined,and it was found that the total flavonoid content of SnCHI transgenic hairy roots was up to 4.14 times that of the blank group.The genetic transformation system of S.nigrum was successfully established,and the function of SnCHI gene was studied,which provided a reference for tissue culture and genetic transformation of the same genus.