文冠果HDAC和HAT基因家族鉴定及表达模式分析OA

Histone deacetylases(HDACs)and histone acetyltransferases(HATs)are a group of reversible enzymes that catalyze histone acetylation/deacetylation,playing crucial roles in plant growth and development.This study aimed to investigate the characteristics of histone deacetylase(XsHDAC)and histone acetyltransferase(XsHAT)family member genes and proteins in Xanthoceras sorbifolium,with a focus on their expression patterns during seed embryo development.Genome-wide identification of XsHDACs and XsHATs family members in X.sorbifolium was conducted.Bioinformatics analyses were performed to examine their physicochemical properties,gene structure,conserved motifs,chromosomal localization,synteny,and cis-acting elements.The expression patterns of XsHDACs and XsHATs genes in different tissues and during seed embryo development were analyzed using quantitative real-time PCR.A total of 15 XsHDACs genes(belonging to the RPD3/HDA1,HD2,and SIR2 subfamilies)and eight XsHATs genes(belonging to the GNAT,MYST,CBP,and TAFⅡ250 subfamilies)were identified from the X.sorbifolium genome.These genes were distributed across nine different chromosomes.Physicochemical analysis of XsHDACs and XsHATs family members revealed that most proteins in both families were hydrophilic and acidic.Subcellular localization predictions indicated that XsHDACs and XsHATs family proteins were primarily localized in the nucleus.Phylogenetic analysis showed a close evolutionary relationship between X.sorbifolium and Litchi chinensis,both belonging to the Sapindaceae family.Protein structure predictions demonstrated that XsHDACs and XsHATs family members were rich in random coils and α-helices.Cis-acting element analysis revealed that the promoters of XsHDACs and XsHATs genes contain elements related to seed development,light response,hormone signaling,and stress responses.Quantitative real-time PCR revealed that most XsHDACs and XsHATs members were highly expressed in the embryo of seed,with increased expression levels during late-stage embryogenesis.The XsHDACs and XsHATs gene families exhibited both evolutionary conservation and specificity.The expression results suggested their potential crucial roles in the embryonic development and oil biosynthesis in X.sorbifolium.