香连化滞丸治疗犬湿热泄泻的机制探索OA

This study aimed to investigate the potential mechanisms of Xianglian Huazhi pills for treating canine dampness-heat diarrhea using a clinical trial integrated with multi-omics analyses.Nineteen diseased dogs were enrolled(10 in traditional Chinese medicine group and 9 in western medicine group),with an additional healthy control group.The two treatment groups were comparable at baseline.After treatment,the total syndrome scores decreased significantly in both groups(P<0.05),and the between-group difference was not significant;however,the TCM group showed a more pronounced reduction in the TCM-specific subscore.The overall response rate was significantly higher(P<0.05)in the TCM group(100.0％)than that in the western medicine group(88.9％).Network pharmacology identified multiple active constituents from the 12 herbal components and constructed a"compound-target-pathway"network,highlighting hub nodes such as AKT1,STAT3,TP53,CASP3,EGFR and MTOR,enriched in PI3K-Akt,TNF,inflammatory bowel disease and related pathways.Fecal 16S rDNA sequencing showed that disease was associated with decreases in beneficial taxa(e.g.Firmicutes)and increases in opportunistic pathogens,whereas TCM treatment restored anaerobic fermenters including Blautia,Ruminococcus groups,and Mediterraneibacter,shifting the community toward a healthy profile.Untargeted metabolomics indicated that disease mainly perturbed lipid and fatty-acid metabolism;TCM treatment enhanced nucleotide/carbohydrate metabolism and cofactor biosynthesis,increased short-chain fatty acids and antioxidant-related small molecules,and reduced markers of oxidative stress.Spearman correlation analyses revealed positive associations between fermentative probiotics and SCFAs(butyrate,valerate,caproate)as well as γ-glutamyl-cysteine and carnosine,while Enterococcus and Acinetobacter were negatively correlated.Collectively,the evidence suggests that Xianglian Huazhi pills act via a multi-component,multi-target,multi-pathway strategy to remodel the gut microecology and metabolic networks,suppress inflammation and oxidative stress,maintain mucosal barrier function,and thereby improve the clinical state of dogs with dampness-heat diarrhea.