鹿茸菇多糖的提取工艺优化及其体外抗氧化作用研究OA
Optimization of extraction process for polysaccharides from Lyophyllum decastes by orthogonal test and their antioxidant activity in vitro
为实现鹿茸菇多糖的高效制备,本研究系统比较了水提法、酶解辅助水提醇沉法、微波水提法、超声水提法及冻融水提法对鹿茸菇多糖提取率与多糖含量的影响.在单因素试验基础上,通过正交试验法优化鹿茸菇多糖提取工艺,并通过DPPH·和ABTS+·清除试验探究该多糖的抗氧化活性.结果显示,水提法、酶解辅助水提醇沉法、微波水提法、超声水提法、冻融水提法多糖提取率分别为(4.63±0.40)%、(19.38±1.90)%、(7.32±0.47)%、(8.57±0.48)%、(11.46±0.50)%,多糖含量分别为(41.87±1.39)%、(77.03±1.76)%、(44.78±0.76%)、(42.57±1.36)%、(54.59±2.22)%,其中酶解辅助水提醇沉法效果最佳,故选用酶解辅助水提醇沉法提取鹿茸菇多糖.在此基础上,通过单因素与正交试验对酶解时间、酶浓度、固液比及提取次数4个关键参数进行优化,确定最佳工艺条件为提取3次,酶解时间150 min,酶浓度2.5%,固液比1∶20(g∶mL).在此工艺下进行3次重复验证试验,鹿茸菇多糖含量分别为83.19%、82.08%和81.96%,多糖提取率分别为20.18%、21.34%和20.96%,工艺稳定可靠.体外抗氧化活性检测发现,由此制备的鹿茸菇多糖对DPPH·和ABTS+·均表现出良好的自由基清除活性,且清除效果呈现剂量依赖关系,质量浓度为4.0 mg/mL的鹿茸菇多糖对两种自由基的清除率分别高达80.17%和88.42%.综上所述,本研究建立了一种高效的鹿茸菇多糖提取工艺,并证实该多糖具有显著的抗氧化潜力,为鹿茸菇在功能性食品中的开发与应用提供了重要依据.
For the efficient preparation of Lyophyllum decastes polysaccharides(LDPs),this study systematically compared the effects of five extraction methods—hot water extraction(HWE),enzyme-assisted aqueous extraction with alcohol precipitation(EAAE),microwave-assisted water extraction(MAE),ultrasound-assisted water extraction(UAE),and freeze-thaw-assisted water extraction(FTE)—on the extraction yield and polysaccharide content.The results showed the following respective yields and polysaccharide contents:HWE:(4.63±0.40)%and(41.87±1.39)%;EAAE:(19.38±1.90)%and(77.03±1.76)%;MAE:(7.32±0.47)%and(44.78±0.76)%;UAE:(8.57±0.48)%and(42.57±1.36)%;FTE:(11.46±0.50)%and(54.59±2.22)%.Among these,EAAE demonstrated the best performance and was therefore selected for LDPs extraction.Subsequently,based on single-factor experiments,the extraction process was optimized using an orthogonal array design focusing on four key parameters:extraction cycles,enzymatic hydrolysis time,enzyme concentration,and solid-liquid ratio.The optimal conditions were determined as follows:3 extraction cycles,150 min enzymatic hydrolysis time,2.5%enzyme concentration,and a solid-liquid ratio of 1∶20(g∶mL).Under these optimized conditions,triple validation experiments confirmed the process's stability and reliability,showing polysaccharide contents of 83.19%,82.08%,and 81.96%,and extraction yields of 20.18%,21.34%,and 20.96%,respectively.Furthermore,in vitro antioxidant activity assays revealed that the prepared LDPs exhibited potent scavenging abilities against both DPPH·and ABTS+·in a dose-dependent manner.At a concentration of 4.0 mg/mL,the scavenging rates reached 80.17%for DPPH·and 88.42%for ABTS+·.In conclusion,this study successfully established an efficient extraction process for LDPs and confirmed their significant antioxidant potential,providing a crucial foundation for the development and application of Lyophyllum decastes in functional foods.
李艳华;周淑棉;何颖;胡庭俊
广西大学动物科学技术学院,广西 南宁 530004贵港市动物疫病预防控制中心,广西 贵港 537100广西壮族自治区兽医研究所 广西兽医生物技术重点实验室,广西 南宁 530001广西大学动物科学技术学院,广西 南宁 530004
农业科技
鹿茸菇多糖正交试验抗氧化活性
Lyophyllum decastes polysaccharidesorthogonal testantioxidant activity
《中兽医医药杂志》 2026 (1)
7-13,7
国家自然科学基金资助项目(32560871)广西研究生教育创新计划项目(YCSW2025084)南宁市科学研究与技术开发计划项目(20232039)
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