中药炮制辅料米泔水质量标准研究OA
Study on Quality Standard of Rice Water of Processing Materials of Traditional Chinese Medicine
目的 提高并完善米泔水质量标准,以保证米泔水炮制中药饮片的质量稳定.方法 增加了米泔水澄清度及pH值测定;采用TLC法,以L-赖氨酸为对照品,对米泔水进行薄层定性鉴别;建立紫外分光光度法测定米泔水中总氨基酸含量及酶活力.结果 10批米泔水浊度值为2.280～3.412;pH值在6.14～6.58;薄层定性鉴别斑点清晰;总氨基酸在5.31～53.1μg(r=0.999 0)范围内线性关系良好,加样回收率97.54％～100.1％(RSD=0.9％);总氨基酸含量在1.19～1.47 mg·mL-1,酶活力在5.42～6.37 U·L-1.结论 提高并完善了米泔水的质量控制方法,方法准确稳定,重复性好,可用于米泔水的质量控制.
Objective To improve and perfect the quality standards of rice water to ensure the stable quality of rice water pro-cessed traditional Chinese medicine decoction pieces.Methods The clarification and pH value of rice water were determined.U-sing L-lysine as the control substance,thin layer chromatography(TLC)was used to qualitatively identify the rice water.The to-tal amino acid content and enzyme activity were determined by ultraviolet-visible spectroscopy(UV)method.Results The tur-bidity values of the 10 batches of samples ranged from 2.280-3.412.The pH values of the 10 batches ranged from 6.14-6.58.The TLC spots were clear and repeatable.The linear range of the total amino acid was 5.31-53.1 μg(r=0.999 0),and the recoveries were 97.54％-100.1％(RSD=0.9％).The total amino acid content was 1.19-1.47 mg·mL-1,and the en-zyme activity was 5.42-6.37 U·L-1.Conclusion The quality control method of rice water was improved.The method is accu-rate,stable and reproducible,and can be used for the quality control of rice water.
王光函;鞠俭奎;辛旭阳;邹桂欣;姜鸿;范英兰;靖博宇;陶弘武
辽宁中医药大学附属第二医院,辽宁沈阳 110034辽宁中医药大学附属第二医院,辽宁沈阳 110034辽宁中医药大学附属第二医院,辽宁沈阳 110034辽宁中医药大学附属第二医院,辽宁沈阳 110034辽宁中医药大学附属第二医院,辽宁沈阳 110034辽宁中医药大学附属第二医院,辽宁沈阳 110034辽宁中医药大学附属第二医院,辽宁沈阳 110034辽宁中医药大学附属第二医院,辽宁沈阳 110034
医药卫生
米泔水质量标准L-赖氨酸总氨基酸酶活力
rice waterquality standardL-lysinetotal amino acidenzyme activity
《中华中医药学刊》 2026 (1)
30-33,后插7,5
国家"十三五"重大新药创制项目(2017ZX09301019)辽宁省教育厅面上项目(LJKMZ20221331,LJKZ0904,JYTMS20231828)辽宁省科技计划联合计划项目(2023-MSLH-151)辽宁中医药大学附属第二医院"育苗工程"项目(2023-LZYY-1-17)
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