我国烟豆种质资源搜集取样策略研究OA

Glycine tabacina(Labill.)Benth.,a perennial species of the subgenus Glycine,is endemic to Fujian province,China,and was listed as a National Grade Ⅱ Key Protected Wild Plant.Rational population sampling to maximize the preservation of genetic diversity is crucial for germplasm collection of this species.In this study,species-specific SSR markers were employed to analyze both inter-population genetic structures across 13 natural populations and intra-population genetic structures in a single-population.The results show that geographically there were extremely low gene differentiation(Fs=0.056),high expected heterozygosity(He=0.741),high polymorphism information content(PIC=0.70),and substantial gene flow(Nm=4.445);intra-population variation(Hs accounting for 78.0％of the total variation)exceeded inter-population differentiation(Dst accounting for 22.0％of the total variation).The results of this study clearly support that:although G.tabacina is a self-pollinating plant,its geospatial population genetic variation characteristic resembles that of outcrossing plants,which may be attributed to the species' perennial nature facilitating high genetic heterogeneity and extensive long-distance dispersal enabling high geographical gene flow.The fine-scale spatial genetic structure analysis within the single population(Longhai)with higher-level disturbance revealed that the size range of spatial genetic patches among plants was about 5.0 m.When the sampling size was 20-25 plants,95％of the population's genetic diversity was captured.Based on the findings of this study,we propose the following strategies for collecting G.tabacina germplasm resources:at the population level,at least 20-25 individual plants should be sampled within a population,with an interval of at least 9-15 m between sampled plants.At the geographical spatial level of populations,since G.tabacina in China is only distributed in a narrow coastal area of Fujian province,it is recommended to collect as many populations as possible.