茅苍术Aux/IAA基因家族鉴定及其对生长素的响应分析OA

Using the whole-genome data of Atractylodes lancea,members of the auxin(Aux)/indole-3-acetic acid(IAA)gene family were identified and characterized,and the effects of 1-naphthaleneacetic acid(NAA)treatment on the expression levels of this gene family were investigated,providing a foundation for further studies on the functions of the A.lancea Aux(AlAux)/IAA gene family.Members of the AlAux/IAA gene family were identified from the A.lancea whole-genome data using TBtools-Ⅱ.Bioinformatics software was employed to analyze the physicochemical properties of the encoded proteins,construct a phylogenetic tree,and predict conserved motifs and gene structures.Tissue-cultured seedlings of A.lancea were used as experimental materials,and key genes of the AlAux/IAA gene family were screened by transcriptome sequencing.The expression of key AlAux/IAA genes was analyzed after one month of NAA treatment.A total of 53 AlAux/IAA genes were identified from the A.lancea genome.Most AlAux/IAA proteins were localized in the nucleus,lacked transmembrane domains,and exhibited negative hydrophobicity.Among them,44 AlAux/IAA genes were predicted to encode functional Aux/IAA transport proteins.Transcriptome sequencing and RT-qPCR analyses showed that the AlAux/IAA family genes exhibited spatiotemporal expression specificity,and different members displayed varying response times and intensities to NAA treatment.Tissue-specific expression analysis revealed that the AlAux/IAA gene family was generally highly expressed in flowers.This study identified the Aux/IAA gene family members in A.lancea,preliminarily analyzed their sequence characteristics,and examined their expression features in response to Aux.It is speculated that AlAux/IAA13 and AlAux/IAA38 may be involved in rooting and bud growth and development during the subculture process of A.lancea tissue-cultured seedlings.These results provide a theoretical basis for elucidating the molecular mechanisms of AlAux/IAA gene function and Aux-mediated regulation of rhizome growth in A.lancea.