基于孟德尔随机化探究情绪波动与肝硬化相关性及中药预测OA

Objective To investigate the causal relationship between mood swings and liver cirrhosis;To predict the potential regulatory effect of Chinese materia medica;To provide ideas for the TCM treatment of liver cirrhosis.Methods With publicly available genome-wide association study(GWAS)data,a two-sample,bidirectional,multivariate Mendelian randomization(MR)approach was employed to examine the causality between mood swings and liver cirrhosis and to assess heterogeneity and horizontal pleiotropy.Single nucleotide polymorphisms(SNPs)were queried to identify effective chemical components for Chinese materia medica prediction.The properties,tastes,meridian tropism and efficacy of these Chinese materia medica were statistically analyzed.A network of target-active component-Chinese materia medica was constructed using Cytoscape software,with core Chinese materia medica identified via the Centiscape and MCODE plugins.Results MR analysis revealed a positive causal relationship between mood swings(exposure)and liver cirrhosis(outcome)(IVW OR=2.361 1,95%CI:1.106 0-5.040 4).There was no heterogeneity,level pleiotropy and outliers among SNPs,and the sensitivity was good.Two-way MR analysis showed that there was no two-way causal relationship between mood swings and liver cirrhosis.Multivariate MR analysis showed that there was a positive causal relationship between mood swings and liver cirrhosis when considering the confounding factors of viral hepatitis and autoimmune hepatitis.A total of 28 key targets were identified,yielding 35 active chemical components,mapping relationship with 640 kinds of Chinese materia medica.The main properties were warm,cold and neutral;the main tastes were sweet,bitter and pungent;the meridian tropism primarily involved the liver,lung and spleen meridians;the primary efficacy included clearing heat-toxicity,cooling the blood and stanching bleeding,and promoting diuresis to alleviate edema.Network analysis identified Curcumae Radix,Curcumae Longae Rhizoma and Ganoderma as the core Chinese materia medica.Conclusion This study suggests that mood swings may increase the risk of liver cirrhosis and identifies Curcumae Radix,Curcumae Longae Rhizoma and Ganoderma as potential core Chinese materia medica,providing references for TCM treatment and new drug development for liver cirrhosis.