X射线对储存血小板及其线粒体的影响OA

Objective To investigate the effects of clinical routine X-ray irradiation dose(average irradiation dose:29.7±0.54 Gy)on the function,apoptosis,activation state and mitochondrial function of platelets during in vitro storage,so as to provide experimental evidence for optimizing platelet irradiation strategies.Methods A paired experimental design was adopted.Platelets were collected from 12 healthy donors,and each sample was equally divided into the irradiated group and the control group(non-irradiated).All samples were stored for 5 days under standard platelet preservation conditions(22±2℃,continuous oscillation).Flow cytometry was used to detect platelet count,apoptosis rate(Annexin V+positive rate),activation markers(CD62P,PAC-1,CD42b)and reactive oxygen species(ROS)level.Meanwhile,mitochondrial-specific probes were used to evaluate changes in mitochondrial count,membrane potential and adenosine triphosphate(ATP)con-tent.Additionally,transmission electron microscopy(TEM)was employed to observe the ultrastructure of platelets,with a focus on mitochondrial morphology,platelet membrane integrity and granule distribution.Results Within 5 days of stor-age,the platelet count was(841±89.16)×109/L in the irradiated group and(824.5±92.88)×109/L in the control group,with no statistically significant difference between the two groups(P=0.54).The apoptosis rate was(4.94±1.39)%in the irradiated group and(5.50±0.83)%in the control group,showing no significant difference(P=0.31).For activation in-dicators,the CD62P expression rate was(24.32±7.57)%in the irradiated group versus(25.21±8.13)%in the control group(P=0.43).The PAC-1 positive rates were(12.15±4.43)%and(11.75±3.40)%in the irradiated group and con-trol group,respectively(P=0.44).The CD42b expression rates were(12.14±4.43)%and(11.75±3.4)%in the two groups,respectively(P=0.47).The ROS levels were(31.98±8.1)%and(30.64±5.89)%in the two groups,respec-tively(P=0.45).No significant differences were found in the above indicators.For mitochondrial function indicators,the mitochondrial count was(55.88±11.49)%in the irradiated group and(53.5±7.24)%in the control group(P=0.57).The ATP contents were(42.45±5.29)%and(41.58±9.50)%in the irradiated group and control group,respectively(P=0.77).The relative membrane potential values were(59.53±10.89)%and(57.49±6.54)%in the two groups,respectively(P=0.47).No significant difference were observed on the mitochondrial function-related indicators.TEM fur-ther confirmed that the ultrastructure of platelets in the irradiation group was intact,the mitochondrial morphology was nor-mal,and no pathological changes such as swelling or vacuolization were observed.Conclusion This study evaluated the impact of conventional-dose X-ray irradiation on platelet storage quality,confirming that this dose does not significant impair platelet count,apoptosis rate,activation status,or mitochondrial function.This finding provides important experimental evi-dence for the clinical promotion of X-ray irradiation technology and suggests its potential as a safe alternative to γ irradia-tion.Future studies could further expand the sample size and extend the observation period to verify the effects of X-ray irra-diation on long-term platelet storage and post-transfusion in vivo survival rate.