小麦TaBCAT-3A、TaBCAT-3B、TaBCAT-3D基因非生物胁迫应答分析OA

Branched-chain amino acid aminotransferase(BCAT)is a key enzyme in the metabolism of branched-chain amino acids,and plays a vital role in plant growth,development and stress adaptation.The bioinformatics analysis was used to identify 3 homologous genes in wheat,TaBCAT-3A,TaBCAT-3B and TaBCAT-3D,which were orthologs of rice OsDIAT gene.These genes exhibited high similarity to OsDIAT in terms of gene structure,amino acid sequences and protein characteristics.Subcellular localization analysis revealed that TaBCAT-3A,TaBCAT-3B,TaBCAT-3D proteins primarily located in cytoplasm and chloroplasts.Promoter cis-element analysis indicated that the promoter regions of TaBCAT-3A,TaBCAT-3B and TaBCAT-3D were enriched with elements responsive to hormones and abiotic stresses.Notably,the promoter region of TaBCAT-3A contained unique defense and stress-responsive elements,further supporting its potential role in plant stress adaptation.Expression analysis demonstrated that TaBCAT-3A,TaBCAT-3B,TaBCAT-3D genes exhibited significant responses under various abiotic stresses,including low temperature,drought,high temperature and salt stress,with pronounced upregulation under low-temperature and salt stress conditions.Comparative expression analysis under drought stress in 3 drought-sensitive and 3 drought-tolerant wheat materials revealed contrasting expression patterns.In drought-tolerant materials,the expressions of TaBCAT-3A,TaBCAT-3B,TaBCAT-3D were significantly downregulated,whereas TaBCAT-3A expression in drought-sensitive materials was significantly upregulated,with no significant changes in TaBCAT-3B and TaBCAT-3D.In conclusion,TaBCAT-3A,TaBCAT-3B,TaBCAT-3D genes were responded under various abiotic stress conditions,highlighting the potential role of TaBCAT-3A in drought stress response.Above results provided new theoretical insights and practical guidance for wheat breeding improved stress resistance.