豇豆种质资源的KASP分子标记鉴定与遗传分析OA

Cowpea[Vigna unguiculata(L.)Walp.]is a major leguminous vegetable in China.Studies on genotype identification and genetic diversity of its germplasm resources are of great significance for the conservation,exploration,and utilization of genetic resources.In this study,46 KASP molecular markers were used to detect and analyze 202 cowpea accessions.The results showed that the average gene diversity(GD)was 0.39(with a variation range of 0.03-0.52),the polymorphism information content(PIC)value was 0.32(with a variation range of 0.03-0.42),and the average major allele frequency(MAF)was 0.69(with a variation range of 0.50-0.99).This indicates that the genetic diversity of the tested cowpea materials is moderately low,which may restrict breeding potential.STRUCTURE analysis divided them into 3 subpopulations with relatively close genetic distances(0.01-0.02).Neighbor-joining(NJ)clustering classified all the cowpea accessions into 4 groups,among which group Ⅰ is mainly local varieties and group Ⅱis mainly commercial varieties.Based on these KASP markers,this study constructed molecular fingerprints of 202 cowpea germplasms,revealed the main SNP composition types at KASP variation sites,and screened 18 representative cowpea germplasms as mini-core germplasm resources,providing a scientific basis for the molecular conservation and utilization of cowpea resources.