光强和外源葡萄糖对黄瓜嫁接苗愈合的协同作用OA

To investigate the effects of light intensity and exogenous spraying of glucose on the healing and photosynthesis of grafted cucumber seedlings,cucumber scions grafted onto squash rootstocks were used as experimental materials.During the graft healing period,different light intensities were set in stages:0,50,and 100 μmol·m-2·s-1 for days 1-3,4-6,and 7-9 after grafting,respectively,with water spraying as the control(CK).Based on this,three treatments were set:spraying 0.5%glucose under CK light conditions(S),increasing the light intensity to 50,100,and 150 μmol·m-2·s-1 in the three stages with water spraying(T),and increasing the light intensity while spraying 0.5%glucose(ST).The results showed that,compared with the control,increasing light intensity and exogenous glucose spraying(ST)increased the net photosynthetic rate and actual photochemical quantum efficiency Y(Ⅱ)of the cotyledons of both the rootstock and scion,significantly improving the photosynthetic performance of the grafted seedlings.The combined treatment of increased light intensity and glucose application promoted the formation of the isolation layer and vascular bundles at the graft union,accelerated xylem connectivity,and shortened the healing process 1-2 days.In conclusion,increasing light intensity and exogenous application of 0.5%glucose during the healing period of grafted seedlings can effectively enhance the photosynthetic performance of cucumber/squash grafting seedlings,promote grafting success,and accelerate the healing process,providing references for environmental regulation and quality improvement in grafted seedling cultivation.