花粉磁转染介导的植物遗传转化技术及其在蔬菜中的应用OA

Transgenetic technologies are crucial for the improvement of crop agronomic traits,yield,and quality.However,conventional transgenic methods face multiple limitations in biological breeding of vegetable crops.Through the method of pollen magnetofection,the exogenous nucleic acids can be directly delivered into pollen grains,and the transformation can be realized by pollination-fertilization.Due to its easy operation and tissue culture-free,pollen magnetofection is thought as one of new transformation techniques with promising application prospects.In this review,the technical principles,application advantages,practical implementation,and current challenges of pollen magnetofection in the transform of vegetable crops,are systematically introduced,and its future improvements are suggested,in order to provide a reference for efficient biological breeding in vegetable species.