口蹄疫病毒3D蛋白与宿主互作蛋白的筛选及鉴定OA
Screening and identification of host proteins interacting with 3D protein of foot-and-mouth disease virus
口蹄疫是由口蹄疫病毒(FMDV)引起的猪、牛、羊等偶蹄类动物急性、热性,高度接触性传染病.FMDV 3D蛋白是病毒编码RNA的RNA依赖性聚合酶(3Dpol),是病毒RNA复制的重要蛋白,有关3D蛋白与宿主蛋白相互作用的报道较少.本研究应用PK-15细胞cDNA酵母表达文库,利用酵母双杂交技术,最终获得与3D有潜在相互作用的15个宿主潜在蛋白,通过免疫共沉淀和共聚焦试验进一步验证,鉴定出宿主ataxin-3和voltage-dependent anion channel 1(VDAC1)与3D具有相互作用,研究结果为宿主蛋白与3D蛋白相互作用在病毒复制中的功能提供了数据支撑.
Foot-and-mouth disease(FMD)is an acute,febrile,and highly contagious infectious dis-ease of cloven-hoofed animals such as pigs,cattle,and sheep,caused by foot-and-mouth disease virus(FMDV).The FMDV 3D protein is the RNA-dependent RNA polymerase(3Dpol)encoded by the virus,which is crucial for viral RNA replication.However,there are few reports on the interaction between 3D and host proteins.In this study,a yeast two-hybrid system was used with a cDNA yeast expression library from porcine kidney epithelial PK-15 cells,and 15 potential host proteins interacting with 3D were ulti-mately obtained.Further validation by co-immunoprecipitation and confocal microscopy identified that host ataxin-3 and VDAC1 interact with 3D.The findings provide data support for the role of host protein-3D interactions in viral replication.
刘永杰;兰喜;李玉星;张勇;何继军;尚佑军;郑海学
甘肃农业大学动物医学院,甘肃兰州 730070中国农业科学院兰州兽医研究所动物疫病防控全国重点实验室,甘肃兰州 730046中国农业科学院兰州兽医研究所动物疫病防控全国重点实验室,甘肃兰州 730046甘肃农业大学动物医学院,甘肃兰州 730070甘肃农业大学动物医学院,甘肃兰州 730070中国农业科学院兰州兽医研究所动物疫病防控全国重点实验室,甘肃兰州 730046中国农业科学院兰州兽医研究所动物疫病防控全国重点实验室,甘肃兰州 730046
农业科技
口蹄疫病毒3D蛋白酵母双杂交技术蛋白质相互作用
foot-and-mouth disease virus3D proteinyeast two-hybrid techniqueprotein-protein interaction
《中国兽医科学》 2026 (1)
13-19,7
国家重点研发计划项目(2021YFD1800300)国家生猪技术创新中心先导科技项目(NCTIP-XD/C03)甘肃省科技特派员专项(24CXNA055)
评论