猫传染性腹膜炎病毒对猫免疫功能的影响及甘露消毒丹和虫草素调节机制研究OA

This study aimed to investigate the effects of feline infectious peritonitis virus(FIPV)stimulation on immunity and phosphodiesterase(PDE)related gene expression of peripheral blood mononuclear cells(PBMCs)in cats,accumulating data for the related immune responses caused by FIPV and the clinical application of traditional Chinese medicine.Firstly,feline PBMCs were isolated and extracted,and then divided into three groups:the control group,the low-concentration FIPV group,and the high-concentration FIPV group.The effects of FIPV stimulation on cat kidney cells(CRFK)and PBMCs were observed through flow cytometry and enzyme-linked immunosorbent assay(ELISA).The changes in immune cells,immunoglobulins,and some immune factors under viral stimulation were observed using real-time fluorescence quantitative PCR to detect the effects of different concentrations of cordycepin and Ganlu Xiaodu Micropill(GXM)on the relative expression levels of PDE1B and PDE4B in CRFK cells and PBMCs under FIPV stimulation.ELISA was used to detect the changes in cAMP levels in CRFK cells and PBMCs stimulated by FIPV at different concentrations of cordycepin and GXM.The results showed that low concentrations of FIPV solution exhibited a certain inhibitory effect on CD4+T cells,which turned into a promoting effect at high doses.As the viral load increases,CD8+T cells were significantly inhibited and the CD4+/CD8+ratio increases significantly.Under FIPV stimulation,PBMCs significantly upregulated the IgM levels and downregulated the IgG levels.After stimulation with FIPV,PBMC secretion of TNF-α and IL-4 increased,while IL-2 and IL-6 decreased.Mechanistically,FIPV reduced cAMP levels by downregulating the gene expression of PDE4B and PDE1B,while GXM and cordycepin upregulating the expression of PDE4B and PDE1B and promoting the restoration of cAMP to normal levels.In summary,under the low dose of FIPV stimulation,cellular immunity is stronger than humoral immunity,while at high concentrations,the opposite is true.FIPV can reduce the expressions of PDE4B and PDE1B genes and decrease the secretion of cAMP.GXM and cordycepin can upregulate the expressions of PDE4B and PDE1B genes and promote the return of cAMP to normal levels,which is expected to become clinical FIP treatment drugs.