响应面优化棉花茎杆皮色素提取工艺及抗氧化研究OA

To optimize the extraction process of natural pigments from cotton stalk fibers and analyze their antioxidant activity,the 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging activity,2,2'-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid(ABTS)radical scavenging ability and iron-reducing power were taken as the key indicators.A strategy combining ultrasonic-assisted technology and response surface methodology was employed to optimize the extraction conditions.The results showed that the optimal pigment extraction conditions were as follows:liquid-to-material ratio of 104.036 mL·g-1,ethanol content of 73.462％,ultrasonic temperature of 59.801℃,and extraction time of 100.056 min.Under these conditions,the extracted pigment content was 51.646 mg·g-1.The antioxidant test indicated that this pigment had a strong scavenging ability for DPPH,ABTS free radicals and iron ions,and the half-maximal inhibitory concentration(IC50)for DPPH was 46.201 39 µg·mL-1,while for ABTS the IC50 was 19.542 30 µg·mL-1.The iron-reducing assay test showed that at a pigment content of 1 mg·mL-1,its activity was almost equivalent to that of vitamin C.Above results opened new avenues for the comprehensive utilization of cotton,particularly in resource-efficient applications and food additive development,showing great potential for future application.