不同覆盖对寒地苹果园土壤微生物群落结构和多样性的影响OA

In order to study the effects of different mulching on soil microbial community structure,relative abundance and diversity in apple orchard of cold region,taking the'Longfeng'apple orchard in the cold region as the research abject,the effects analysis of soil bacterial and fungal community structure and diversity under 4 different mulching measures(rice straw,corn straw,edible fungi residue and horticultural fabric)were carried out by using high-throughput sequencing technology,with clear tillage as the control.The results showed that the relative abundance of bacterial Actinobacteriota and fungal Basidiomycota were significantly increased by the treatment of horticultural fabric.The relative abundance of bacterial Proteobacteria,Bacteroidota,Myxomycoccota and fungal Ascomycota were significantly increased by the treatments of corn straw and edible fungi residue.The relative abundance of Gemmatimonadota of bacterial phyla and Basidiomycota,Mortierellomycota of fungi phylum were significantly reduced by the treatments of corn straw and edible fungi residue.The results of Beta diversity analysis showed that the community structure of soil bacteria and fungi was more similar between the treatments of corn straw and edible fungi residue,with significant differences compared to the control.Alpha diversity analysis indicated that the richness and diversity of soil bacterial and fungal communities were significantly improved under edible fungi residue mulching,while the richness of soil bacterial communities significantly improved under horticultural fabric mulching.Four mulching treatments significantly increased the diversity index of soil fungal,while organic mulching treatments significantly increased soil fungal richness.Overall,mulching significantly affected the soil microbial community structure of apple orchards in cold region and inproved diversity of soil microbial.Among them,the best effect of all was edible fungi residue mulching.Above results provided theoretical basis for the research on mulching cultivation techniques in cold region of apple orchards.