大蕉叶化学成分及细胞增殖抑制活性研究OA
Chemical constituents from leaves of Musa×paradisiaca and their anti-proliferative activities
目的 研究大蕉Musa×paradisiaca叶化学成分以及体外细胞增殖抑制活性.方法 采用硅胶、MCI gel CHP-20P、反相ODS和Sephadex LH-20 柱色谱以及半制备型高效液相等色谱方法进行分离和纯化,通过核磁共振、质谱等技术手段鉴定了化合物的结构.利用CCK-8 法评价了所有化合物体外细胞增殖抑制活性.结果 从大蕉叶提取物中分离得到 19 个化合物,分别鉴定为邻苯二甲酸二(2-乙基)己酯(1)、肉豆蔻酸(2)、正十五酸(3)、亚油酸(4)、邻苯二甲酸二丁酯(5)、4-羟基-3-甲氧基苯甲酸乙酯(6)、二氢猕猴桃内酯(7)、MF-EA-705β(8)、邻苯二甲酸丁基酯 2-乙基己基酯(9)、三十四碳-(20,23)-二烯酸(10)、3-羟基豆甾-5,22-二烯-7-酮(11)、3-羟基豆甾-5-烯-7-酮(12)、luffarin X(13)、6-羟基豆甾-4,22-二烯-3-酮(14)、6-羟基豆甾-4-烯-3-酮(15)、(3S,5R,6S,7E)-5,6-环氧-3-羟基-7-巨豆烯-9-酮(16)、对羟基苯甲酸乙酯(17)、对二乙氧基苯(18)、(9Z,12Z,15Z)-十八烷基-9,12,15-三烯酸甲酯(19).化合物 2、5、7、11、13、15 和 17 分别对人肝癌HepG2细胞、人宫颈癌Hela细胞、人乳腺癌MCF-7 细胞和人皮肤恶性黑色素瘤A375 细胞增殖表现出一定的抑制活性,半数抑制浓度(median inhibition concentration,IC50)值为40.26～97.75 μmol/L,其中化合物 7 和 11对A375 细胞增殖有选择性抑制活性(IC50 值分别为 43.0、50.0 μmol/L),略低于阳性对照顺铂(IC50 值 40.13 μmol/L),而化合物 2 和15对MCF-7 细胞增殖选择性抑制活性(IC50 值分别为 40.26、45.00 μmol/L)强于阳性对照(IC50 值为 48.36 μmol/L).结论 化合物1、6～19 为首次从该属植物中分离得到;化合物 2～5 为首次在该植物中分离得到.活性测试表明,化合物 2、7、11 和 15 对特定肿瘤细胞株表现出选择性增殖抑制活性.
Objective To investigate the chemical constituents from the leaves of Musa×paradisiaca and their inhibitory activities on cell proliferation in vitro.Methods The compounds were isolated and purified using various chromatographic techniques,including silica gel,MCI gel CHP-20P,reversed-phase ODS,Sephadex LH-20 column chromatographies,and semi-preparative HPLC.Their structures were elucidated by spectroscopic methods such as NMR and MS.The inhibitory activities of all compounds on cell proliferation in vitro were evaluated using the CCK-8 assay.Results A total of 19 compounds were isolated from the leaves of Musa×paradisiaca and identified as di(2-ethylhexyl)phthalate(1),myristic acid(2),pentadecanoic acid(3),linoleic acid(4),dibutyl phthalate(5),ethyl 4-hydroxy-3-methoxybenzoate(6),dihydroactinidolide(7),MF-EA-705β(8),butyl 2-ethylhexyl phthalate(9),n-tetratriacont-20,23-dienoic acid(10),3-hydroxystigmasta-5,22-dien-7-one(11),3-hydroxystigmast-5-en-7-one(12),luffarin X(13),6-hydroxystigmasta-4,22-dien-3-one(14),6-hydroxystigmast-4-en-3-one(15),(3S,5R,6S,7E)-5,6-epoxy-3-hydroxy-7-megastigmen-9-one(16),ethyl p-hydroxybenzoate(17),p-diethoxybenzene(18),and methyl(9Z,12Z,15Z)-octadeca-9,12,15-trienoate(19).Among them,compounds 2,5,7,11,13,15,and 17 exhibited certain anti-proliferative activities against human hepatocellular carcinoma HepG2,cervical cancer Hela,breast cancer MCF-7,and malignant melanoma A375 cell lines,with median inhibition concentration(IC50)values ranging from 40.26 to 97.75 μmol/L.Specifically,compounds 7 and 11 showed selective inhibitory activity against A375 cells(IC50=43.0 and 50.0 μmol/L,respectively),which was slightly weaker than that of the positive control cisplatin(IC50=40.13 μmol/L).In contrast,compounds 2 and 15 demonstrated stronger inhibitory effects against MCF-7 cells(IC50=40.26 and 45.00 μmol/L,respectively)compared to cisplatin(IC50=48.36 μmol/L).Conclusion Compounds 1 and 6-19 were isolated from this genus for the first time,while compounds 2-5 were first identified from this specific plant.Furthermore,compounds 2,7,11,and 15 exhibited selective inhibitory activities against specific tumor cell lines.
严绪华;孙佳乐;梁秋明;王峰;方振峰
武汉市中医医院 药学综合部,湖北 武汉 430010江汉大学医学部 药学系,湖北 武汉 430056国家中医药管理局中药数字化质量评价技术重点研究室,广东 广州 510006广东省中药质量工程技术研究中心,广东 广州 510006国家中医药管理局中药数字化质量评价技术重点研究室,广东 广州 510006
医药卫生
芭蕉属大蕉叶细胞增值抑制活性邻苯二甲酸二(2-乙基)己酯二氢猕猴桃内酯3-羟基豆甾-5,22-二烯-7-酮6-羟基豆甾-4-烯-3-酮(9Z,12Z,15Z)-十八烷基-9,12,15-三烯酸甲酯
Musa L.leaves of Musa×paradisiaca L.anti-proliferative activitydi(2-ethylhexyl)phthalatedihydroactinidolide3-hydroxystigmasta-5,22-dien-7-one6-hydroxystigmast-4-en-3-onemethyl(9Z,12Z,15Z)-octadeca-9,12,15-trienoate
《中草药》 2026 (3)
817-824,8
广东省普通高校重点领域专项(2022ZDZX2035)
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