小儿复方鸡内金咀嚼片指纹图谱及治疗泄泻的药效学研究OA

Objective To establish HPLC fingerprint detection method of child compound endothelium corneum(CCEC)chewable tablet,and to study its effect on Dahuang induced spleen deficiency diarrhea model in mice and irritable bowel syndrome with diarrhea(IBS-D)model in rats.Methods The fingerprint of CCEC was established by HPLC,and the peak of the fingerprint chromatogram was classified and the peak location was studied.Using the Software System for Similarity Evaluation of TCM Chromatographic Fingerprint issued by the National Pharmacopoeia Committee,15 batches of HPLC chromatographic peaks were automatically matched to form a common pattern map,the common peaks were calibrated,and the control map R was established,and the similarity was calculated with the control map R.A rat model of spleen deficiency diarrhea induced by Dahuang was used to investigate the effects of CCEC on D-xylose absorption,gastric emptying and intestinal propulsion.A rat model of IBS-D was used to investigate the effects of CCEC on the number of loose stools and intestinal propulsion.Results The fingerprint of CCEC was established,and the chromatographic peaks in it were attributed.A total of 6 chemical components were identified through the peak localization study.At the same time,the common pattern map and the control map R of 15 batches of fingerprints were established,and a total of 17 common peaks were labeled.The similarity between the 15 batches of samples and the control spectrum R was greater than 0.95.In the study of the effect of CCEC on spleen deficiency diarrhea rat model,compared with the model group,the number of defecation in Bupi Xiaoji oral liquid group and CCEC dose group was significantly reduced(P<0.01).Compared with model group,the content of D-xylose in Bupi Xiaoji oral liquid group,CCEC high dose group and CCEC medium dose group was increased(P<0.05),the gastric emptying rate was decreased(P<0.05),and the intestinal propulsion rate was decreased(P<0.05).In the study of the effect of CCEC on IBS-D rat models,the number of loose stools and intestinal propulsion rate in the trimebutine group and the CCEC high dose group were significantly reduced compared with the model group(P<0.01).Conclusion The HPLC fingerprint detection method established in this study is convenient,accurate and reproducible,and can evaluate the quality of CCEC comprehensively and effectively.CCEC can improve D-xylose absorption and inhibit gastric emptying and intestinal propulsion in mice with spleen deficiency diarrhea.It can inhibit the number of loose stools and intestinal propulsion in IBS-D rats.The pharmacodynamic study of CCEC shows that it has the effect of treating diarrhea and IBS-D,which is consistent with its clinical application.This study provides a certain pharmacodynamic basis for its clinical application in the treatment of diarrhea.