基于TCGA数据库分析RCN1在肝细胞癌中的表达特征和生物学功能OA

Objective To investigate the expression level of RCN1 in patients with primary hepatocellular carcinoma(HCC)and analyze its relationship with the clinicopathological features and prognosis,and to explore its possible biological functions.Methods The expression of RCN1 in liver cancer was analyzed in TCGA and UALCAN databases.TCGA Liver hepatocellular carcinoma(LIHC)data were visualized by R language to clarify the relationship between RCN1 expression and clinical stage and grade of liver cancer patients.ROC curve and Kaplan-Meier method were used to evaluate the value of RCN1 in the diagnosis and prognosis of liver cancer patients.The differentially expressed and co-expressed genes of RCN1 in patients with liver cancer in TCGA were analyzed by LinkedOmics platform,and the overlapping genes were enriched by GO and KEGG analysis.Results TCGA database showed that RCN1 was highly expressed in clinical samples of HCC patients(P<0.01).The expression of RCN1 was associated with pathological TNM stage,grade,survival and prognosis of patients(P<0.05).RCN1 was up-regulated in differential genes.GO and KEGG analysis showed that RCN1 gene was mainly involved in the activation of nuclear receptors and the activation of transcription factors.Through PPI network analysis,central targets such as ESR1 were found.And through correlation analysis,the interaction between RCN1 and ESR1 might be involved in the regulation of endoplasmic reticulum stress and induce the malignant progression of liver cancer cells.Conclusion RCN1 is highly expressed in hepatocellular carcinoma,and its expression is associated with TNM stage and survival prognosis of hepatocellular carcinoma.While,RCN1 may be related to the activation of nuclear receptors and transcription factors,and regulate the malignant behavior of hepatocellular carcinoma cells together with ESR1 and other genes.