GTP环化水解酶1与冠状动脉性心脏病的关联及其转录表达水平OA

Objective To investigate the causal relationship between GTP cyclohydrolase 1(GCH1)and coronary artery heart disease(CHD)and its transcriptional expression level.Methods The causal relationship between GCH1 and CHD was determined by Mendelian randomization(MR).The standardized mean difference(SMD)of GCH1 mRNA expression level was calculated by integrating CHD-related multi-center high-throughput data.The clinical application value of GCH1 in CHD was evaluated by summarizing the receiver operating characteristic curve,calculating sensitivity,specificity,likelihood ratio and other indicators.Results Forward MR method showed that GCH1 was significantly associated with CHD(OR=0.95,95%CI=0.91-0.99,P=0.021).Bayesian weighted MR method supported this association(OR=0.95,95%CI=0.91-0.99,P=0.025).Backward MR method showed that there was no significant correlation between GCH1 and CHD(OR=1.06,95%CI=0.99-1.13,P=0.104).In addition,a total of 676 CHD and 459 normal control samples were included in this study.The expression of GCH1 was significantly down-regulated in CHD,and its SMD was-0.18(95%CI:-0.31 to-0.06),the area under the summary receiver operating characteristic curve was 0.72(95%CI:0.67-0.75),the sensitivity was 0.61(95%CI:0.48-0.72),the specificity was 0.71(95%CI:0.62-0.79),the positive likelihood ratio was 2.08(95%CI:1.72-2.52),and the negative likelihood ratio was 0.56(95%CI:0.44-0.70).Conclusion GCH1 gene may play an important role in inhibiting the progression of CHD as an inhibitor.