甘蓝型油菜EMS突变体库特异脂肪酸构成材料苗期耐寒性评价及其基因挖掘OA

Rapeseed is an important source of edible oil and forage protein in China.Improving seed oil content,optimizing fatty acid composition,and enhancing cold tolerance are crucial goals in rapeseed breeding.This study utilized an Ethylmethanesulfonate(EMS)mutant library of Brassica napus'Zhongshuang 11'constructed with 1%EMS mutagenesis.After two consecutive self-crosses com-bined with quality screening,259 germplasm lines with specific fatty acid profile were obtained.Subse-quently,19 M4 materials that survived under the prolonged low temperatures during the 2022-2023 overwintering period and produced sufficient seeds were evaluated for cold tolerance.The study found that two cold-tolerant germplasms exhibited enhanced enzymatic scavenging capabilities compared to two cold-sensitive germplasms,resulting in reduced levels of reactive oxygen species and malondialde-hyde.Further transcriptome sequencing of the cold-tolerant germplasm 3Y1210-4#1 and the cold-sen-sitive germplasm 3Y1353-2#4 only identified 2 609 low-temperature-responsive differentially ex-pressed genes(DEGs).GO enrichment analysis of these DEGs revealed that they are broadly involved in cold response,fatty acid synthesis,hormone regulation,oxidative stress,and osmotic stress.