葡萄皮渣中白藜芦醇提取工艺优化及抗氧化活性初步研究OA

The experiment was to study optimal extraction process andantioxidant activity of resveratrol from grape peel residue by supercritical CO2.The extraction rate of resveratrol was used as the investigation index,extraction pressure,extraction time,extraction temperature and entrainer flow rate were used as the single factors,Box-Behnken design and response surface were used to optimize the extraction process condi-tions.The antioxidant activity of extract was analyzed in terms of 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical clearance and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate(ABTS)radical cation clearance.Results showed that the optimum extraction conditions were as follows:extraction pressure of 25 MPa,extrac-tion time of 1.7 h,extraction temperature of 45℃and entrainer flow rate of 0.7 mL/min.Under these conditions,the yield of resveratrol was 9.09％.The antioxidant test showed that the extract from grape peel residue had strong antioxidant capacity.The scavenging rates of resveratrol on DPPH and ABTS free radicals was concentration-dependent,and the IC50 was 0.52 mg/mL and 0.40 mg/mL,respec-tively.Therefore,extraction of resveratrol from grape peel by supercritical CO2 is stable and fea-sible,and the extractive is very potential as feed additives for animals breeding.