BAP31对急性炎症中M1型巨噬细胞的影响及其机制分析OA

Objective:To investigate the regulatory effect of B cell associated receptor protein 31(BAP31)on M1 macrophages in acute inflammation.Methods:Lyz2Cre-BAP31flox/flox and BAP31flox/flox mice were intraperitoneally injected with lipopolysaccharide(LPS)to establish an acute inflammation mouse model,and the effects of LPS on body weight and colon length of each group were evaluated.Bone marrow cells were extracted from the femurs of BAP31flox/flox and Lyz2Cre-BAP31flox/flox mice for in vitro experiments.After induction into macrophages using 10%L929 mouse fibroblast supernatant,the expression of genes and proteins related to macrophage polarization was detected by RT-qPCR and Western blot.Results:In the acute inflammation model,compared with Lyz2Cre-BAP31flox/flox mice injected with PBS,Lyz2Cre-BAP31flox/flox mice injected with LPS for 72 h showed decreased body weight and significantly shortened colon length.There was a large amount of inflammatory cell infiltration and significant glandular atrophy in the intestinal mucosa.In vitro experiments showed that bone marrow-derived macrophages from Lyz2Cre-BAP31flox/flox mice,compared to those from BAP31flox/flox mice,showed significantly increased expression of M1 macrophage-related genes after stimulation with LPS,IFNγ,or LPS+IFNγ,while M2 macrophage-related genes was significantly decreased.Western blot results showed that BAP31 knockdown upregulated the protein expression levels of p-STAT1 and p-ERK.Conclusion:Knockdown of BAP31 affects M1 macrophage activation by regulating the phosphorylation levels of STAT1 and ERK,providing a new target for the prevention and treatment of acute inflammation.