低能量饲粮中添加二氯乙酸钾对肉鸡生长性能、血液生化指标与肠道功能的影响OA

The experiment aimed to investigate the effect of supplementation of Potassium dichloroacetate(DCA-K)in low-energy diets on broilers.Two hundred and seventy 1-day-old Cobb 500 broilers were randomly divided into three groups(6 repli-cates per group,15 broilers per replicate),which were control group(CON group,energy level of 12.85 MJ/kg),low-energy group(LME group,energy level of 12.43 MJ/kg),and the low-energy+potassium dichloroacetate group(LME+DCA-K group,energy level of 12.43 MJ/kg,with a DCA-K addition of 0.1％).The experimental period was 35 days.The results showed that the body weight and average daily gain were significantly lower and the feed-to-weight ratio was significantly higher in the LME group compared with the CON group(P<0.05).Calcium(Ca)content in plasma was significantly lower in broilers in the LME group compared to the CON group(P<0.05),and supplementation with DCA-K on the basis of a low-energy diet significantly increased plasma ALT concentration and HDL content and significantly decreased plasma TG and LDL content(P<0.05).The LME group significantly increased duodenal lipase and jejunal trypsin activities in broilers compared to the CON group(P<0.05).The LME+DCA-K group significantly increased duodenal and jejunal trypsin activity and jejunal lipase activity and dialed back duodenal lipase activity compared to the LME group(P<0.05).Compared with the CON group,the LME group significantly down-regulated the mRNA levels of broiler jejunal Mucin-2,SLC1A1,SGLT-1,KCNJ13,LXRα,and FABP4.The LME+DCA-K group significantly up-regulated the mRNA levels of these genes,as well as significantly up-regulated the mRNA levels of SLC7A7(P<0.05).In conclusion,the addition of 0.1％DCA-K to low-energy diets can improve blood biochemical indexes and intestinal barrier function of broilers,as well as enhance intestinal absorption of amino acids and lipids.