磁性分子印迹聚合物固相萃取-超高效液相色谱-串联质谱法测定花生和酱油中4种黄曲霉毒素OA

This study established a new method using magnetic solid-phase extraction(MSPE)based on a novel magnetic molecularly imprinted polymer(Fe3O4@MIP)combined with ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)for the rapid and sensitive determination of four aflatoxins(AFs)in peanuts and soy sauce.Using a surface grafting polymerization strategy,Fe3O4@MIP with selectivity for AFs was successfully prepared with 5,7-dimethoxycoumarin as a dummy template,methacrylic acid(MAA)as a functional monomer,and ethylene glycol dimethacrylate(EGDMA)as a cross-linker.Subsequently,the MSPE extraction conditions were systematically optimized.The results showed that the developed method had good linearity within the concentration range of 0.02–100 μg/kg(R2>0.991 5),with spiked recoveries in the peanut matrix ranging from 87.63%to 103.58%.Both intra-day and inter-day precision(relative standard deviation(RSD))were below 8.16%,and the limit of detection(LOD)was as low as 0.006 9–0.011 μg/kg.The prepared Fe3O4@MIP exhibited excellent selectivity and reusability for AFs.The MSPE-UPLC-MS/MS method is simple to operate and highly sensitive,and can effectively overcome matrix interferences,providing a reliable solution for the rapid and accurate detection of trace AFs in complex foods,and holding significant application value in the field of food safety monitoring.