小麦光腥黑粉菌g5965蛋白的特性分析与功能研究OA

Wheat common bunt is one of the major wheat diseases which caused by Tilletia foetida,not only reducing wheat yield,but also deteriorating the quality of the wheat.To investigate the interaction mechanism between the common bunt fungus T.foetida and Triticum aestivum,a candidate effector protein g5965(GenBank No.XXN76190.1)belonging to the protein kinase superfamily was cloned via RT-PCR.The results of bioinformatics analysis revealed that the g5965 gene had a full length of 1 722 bp,encoded 573 amino acids,and contained a typical phosphotransferase domain.Its signal peptide secretion function had been verified through signal peptide secretion assays.Constructed the PGR107-g5965 fusion expression vector and transiently expressed it in the leaves of Nicotiana benthamiana via Agrobacterium-mediated transformation.The g5965 protein did not suppress the programmed cell death(PCD)response in tobacco cells induced by the pro-apoptotic protein BAX(Bcl-2 associated X protein).The green fluorescent protein(GFP)expression vector pBIN-g5965 was constructed and used to infect N.benthamiana leaves.The green fluorescence was observed at the cell membrane of tobacco cells under a laser scanning confocal microscope after 2～3 d.This study provides a certain theoretical basis for further elucidating the pathogenic mechanism of T.foetida against the wheat.