桦褐孔菌的化学成分及抗过敏活性OA
Chemical constituents and anti-allergic activities of Inonotus obliquus
桦褐孔菌Inonotus obliquus是一种珍贵的食药用真菌,传统医学中常用于治疗糖尿病、高血脂、肝炎等疾病.为进一步开发桦褐孔菌的功效,作者综合采用溶剂萃取和各种色谱技术(反相硅胶、正相硅胶及凝胶LH-20等),从桦褐孔菌中分离得到8个化合物,并根据核磁波谱数据,将这些化合物分别鉴定为原儿茶酸(1)、丁香酸(2)、咖啡酸(3)、4-(3,4-二羟苯基)-3-丁烯-2-酮(4)、桦褐孔菌醇(5)、栓菌酸(6)、羊毛甾醇(7)和过氧麦角甾醇(8).抗过敏活性实验显示,桦褐孔菌醇(5)在4.96、9.92和15.50 μmol/L浓度的脱颗粒率分别为51.03％、30.53％和23.07％,均能显著抑制肥大细胞脱颗粒,具有明显的抗敏舒缓效果.此外,桦褐孔菌醇(5)在 15.50 μmol/L浓度以下未表现出明显的细胞毒性,有望用于开发新型抗敏药物.本文研究结果为桦褐孔菌功能性产品开发提供了重要参考.
Inonotus obliquus is a medicinal and edible fungus traditionally used for treating diabetes,hyperlipidemia,hepatitis,and related disorders.In this study,by use of solvent extraction and chromatographic techniques(reversed-phase silica gel,normal-phase silica gel,and Sephadex LH-20),eight compounds were isolated from I.obliquus.Based on nuclear magnetic resonance spectroscopic data,these compounds were identified as gallic acid(1),syringic acid(2),caffeic acid(3),4-(3,4-dihydroxyphenyl)-3-buten-2-one(4),inotodiol(5),cortinellin(6),lanosterol(7),and peroxide ergosterol(8).Anti-allergic activity assays demonstrated that inotodiol(5)significantly inhibited mast cell degranulation at concentrations of 4.96,9.92,and 15.50 μmol/L,with inhibition rates of 51.03％,30.53％,and 23.07％,respectively,indicating potent anti-allergic efficacy.Notably,inotodiol(5)exhibited unobserved cytotoxicity at concentrations up to 15.50 μmol/L,highlighting its potential as a candidate for novel anti-allergic drug development.
李海燕;张慧敏;陈烜著;吴峰;陈维广;郑永标
福建师范大学生命科学学院,福建 福州 350117福建师范大学生命科学学院,福建 福州 350117福建师范大学生命科学学院,福建 福州 350117福州碧昂缇生物科技有限公司,福建 福州 350108福州碧昂缇生物科技有限公司,福建 福州 350108福建师范大学生命科学学院,福建 福州 350117
桦褐孔菌桦褐孔菌醇羊毛甾醇抗过敏活性
Inonotus obliquusinotodiollanosterolanti-allergic activity
《菌物学报》 2026 (1)
163-174,12
福建省高校产学合作计划(2024N5005) This work was supported by the Fujian Provincial University-Industry Collaboration Program(2024N5005).
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