亚窄孢灵芝中杜松烷型倍半萜及其α-葡萄糖苷酶抑制活性OA

The chemical constituents of Ganoderma subangustisporum and their α-glucosidase inhibitory activity were investigated.Two cadinane-type sesquiterpenes,17-hydroxy-12-ethoxycarbonyl-α-cadinol(1)and 12-hydroxy-α-cadinol(2),were isolated from the ethyl acetate extract of G.angustisporum mycelial cultures.Their structures were elucidated through high-resolution mass spectrometry,comprehensive nuclear magnetic resonance spectroscopic analysis and quantum chemical calculations.Compound 1 is novel due to the acetylation of its 12-hydroxy group,while compound 2 is reported for the first time from the genus Ganoderma.Both compounds 1 and 2 exhibited α-glucosidase inhibitory activity,with IC50 values of(96.72±3.87)μmol/L and(56.82±4.70)μmol/L,respectively.Molecular docking results indicate that compounds 1 and 2 primarily interact with key amino acid residues in the active site of α-glucosidase through hydrogen bonds.