甜味小鬼伞生物学特性、驯化栽培及抗氧化活性OA
Biological characteristics,domestic cultivation and antioxidant activities of Coprinellus saccharinus
本研究以分离自河北省秦皇岛市青龙县南山公园落叶上腐殖层菌丝获得的甜味小鬼伞Coprinellus saccharinus 菌株为试验材料,对生物学特性的 4 个因素进行了研究,成功培育出子实体,并测定菌丝体和子实体醇提物对 ABTS+·和羟自由基的清除能力.结果表明:在生物学特性试验中,甜味小鬼伞菌株的最适碳源是蔗糖和可溶性淀粉,最适氮源是硝酸钾,适宜的 pH 范围为7-9,在 25℃条件下菌丝长速最快;在栽培驯化试验中,温度在(20±1)℃条件下菌丝满瓶需要20-25 d,其后给予 2-4℃温差,保持相对空气湿度 70％-80％,12 h/d光周期 800-1000 lx散射光,约10-15 d后现原基,2-3 d后子实体成熟.甜味小鬼伞菌丝体与栽培子实体醇提物的抗氧化活性均随添加量的增加逐渐增强,对 ABTS+·和羟自由基的清除率均在醇提物添加量为 200 μL 时达到最高.其中,栽培子实体醇提物的清除率分别为97.93％和 67.22％,显著高于菌丝体的相应值(64.73％和24.74％).菌丝体和栽培子实体醇提物对ABTS+·的清除能力均强于羟自由基,其清除率分别相差39.99％和 30.71％.本研究结果为深入探究甜味小鬼伞的栽培技术、生理活性、药理作用及其他开发利用提供了实践依据.
A Coprinellus saccharinus strain was isolated from mycelia on deciduous leaf humus in Nanshan Park(Qinglong County,Hebei,China).Four factors of biological characteristics were investigated.Fruiting bodies of this species were successfully cultivated.The scavenging capacities of both mycelium and fruiting body ethanol extracts against ABTS+·and·OH were also evaluated.The results indicated that the optimum carbon source for C.saccharinus strain were sucrose and soluble starch;the optimum nitrogen source was KNO3;the suitable pH range was 7-9,and the highest mycelial growth rate was observed at 25℃.Domestication experiment showed that bottleful mycelial colonization time required 20-25 d at(20±1)℃;primordium differentiation demanded temperature difference of 2-4℃,relative air humidity at 70％-80％,photoperoid of 12 h/d 800-1000 lx under scattered light,and a duration of 10-15 d.Mature fruiting bodies can be harvested after continued cultivation for 2-3 d.The antioxidant activities of C.saccharinus ethanol extracts from mycelia and cultivated fruiting bodies increased gradually as additive amount raising.Scavenging rates against both ABTS+·and·OH peaked at an additive volume of 200 μL.Scavenging rates of ethanol extract of cultivated fruiting bodies reached 97.93％and 67.22％,respectively,significantly higher than those of mycelia(64.73％and 24.74％).Both mycelium and cultivated fruiting body ethanol extracts exhibited stronger scavenging capacity against ABTS+·than·OH.Scavenging rate differentials were 39.99％and 30.71％,respectively.These findings provided a practical basis for further investigations into cultivation techniques,physiological activities,pharmacological effects,and other utilizations of C.saccharinus.
赵怡;康霞;常光雷;王立安;彭慧芬;田景花;李守勉;李国杰
河北农业大学园艺学院 河北省蔬菜种质创新与利用重点实验室 河北省蔬菜产业协同创新中心,河北 保定 071001河北农业大学园艺学院 河北省蔬菜种质创新与利用重点实验室 河北省蔬菜产业协同创新中心,河北 保定 071001河北农业大学园艺学院 河北省蔬菜种质创新与利用重点实验室 河北省蔬菜产业协同创新中心,河北 保定 071001河北师范大学生命科学学院,河北 石家庄 050024河北农业大学园艺学院 河北省蔬菜种质创新与利用重点实验室 河北省蔬菜产业协同创新中心,河北 保定 071001河北农业大学园艺学院 河北省蔬菜种质创新与利用重点实验室 河北省蔬菜产业协同创新中心,河北 保定 071001河北农业大学园艺学院 河北省蔬菜种质创新与利用重点实验室 河北省蔬菜产业协同创新中心,河北 保定 071001河北农业大学园艺学院 河北省蔬菜种质创新与利用重点实验室 河北省蔬菜产业协同创新中心,河北 保定 071001
药用真菌生长条件人工栽培
medicinal fungusgrowth conditionartificial cultivation
《菌物学报》 2026 (1)
142-153,12
河北省食用菌产业创新团队珍稀食用菌岗位(HBCT2023090202)河北省自然科学基金(C2025204257)河北农业大学引进人才科研专项(YJ201849)国家食用菌产业技术体系小宗种类食用菌栽培岗位(CARS-20-4)河北省县域生物多样性调查评估项目(2011600012)河北省科技支撑计划(2053731D)河北省科技计划项目重点研发计划(21326315D)河北省现代农业产业技术体系食用菌创新团队项目(HBCT2018050205) This work was supported by the Innovation Team of Edible Fungi of Hebei Modern Agricultural Industrial Technology System,Valuable and Rare Edible Fungus Position(HBCT2023090202),the Hebei Natural Science Foundation(C2025204257),the Talent Introduction Scientific Research Special Project of Hebei Agricultural University(YJ201849),the Earmarked Fund for China Agriculture Research System(CARS)-Edible Fungi,Non-staple Edible Fungus Species Cultivation Position(CARS-20-4),the Intra-County Biodiversity Investigation and Evaluation of Hebei Province(2011600012),the Hebei Province Science and Technology Support Plan(2053731D),the Key Research and Development Planning Project in Science and Technology of Hebei Province(21326315D),and the Innovation Team of Edible Fungi of Hebei Modern Agricultural Industrial Technology System(HBCT2018050205).
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