白化卵孢小奥德蘑生物学特性、驯化及抗病性OA

A wild white macrofungi collected from Leye County of Guangxi was identified as albino Oudemansiella raphanipes through morphological observation and molecular analysis.Optimal conditions for mycelial growth,including carbon source,nitrogen source,pH and temperature were determined using single-factor and orthogonal tests.The results showed that the suitable carbon source and nitrogen source were fructose(30 g/L)and yeast extract powder(8 g/L),respectively.The suitable pH and temperature were 8.0 and 25℃respectively.The optimum combination condition for vigorous growth was fructose of 20 g/L,yeast extract powder of 6 g/L,pH of 7.0 and temperature of 25℃.Expanded bag-cultivation trials with 15 cm×26 cm cultivation bag revealed that the bagful mycelial colonization time,in substrate containing 78％sawdust,was about 25 days,and subsequent cultivation for approximately 20 days resulted in appearance of the brown mottlement.The mushroom button arose approximately 60 days after soil-covering soil on the split of upside-down mushroom bag,and the fruiting bodies could be harvested after 4-6 days of subsequent cultivation.The average fresh fruiting body weight was 41.83 g/bag and the average biological efficiency was 16.09％in two fruiting stages.Albino O.raphanipes was susceptible to Trichoderma harzianum,with 33.04％inhibition rate before contact and 95.55％infection rate after contact.This research provides a theory reference for extended development and utilization of an albino O.raphanipes.