Yip1结构域家族成员4通过IER3IP1促进肝细胞癌的恶性进展OA

Objective To investigate the role of Yip1 domain family member 4(YIPF4)in the malignant pro-gression of hepatocellular carcinoma(HCC).Methods Bioinformatics analysis,Western blot,and immunohisto-chemistry(IHC)were used to assess YIPF4 expression in HCC and adjacent non-tumor liver tissues and its association with clinicopathologic parameters.Stable YIPF4-knockdown Hep3B and PLC/PRF/5 cell lines were generated and sub-jected to CCK-8,colony formation,and Transwell assays to evaluate the effects of YIPF4 depletion on HCC cell prolif-eration,migration,and invasion.The cBioPortal and GEPIA2 databases were interrogated to identify genes positively correlated with YIPF4,revealing immediate early response 3 interacting protein 1(IER3IP1).The expression differ-ences of IER3IP1 in HCC and adjacent tissues were detected by bioinformatics and immunohistochemistry methods,and its correlations with the clinicopathological characteristics of patients and YIPF4 were analyzed,and Western blot was used to determine changes in IER3IP1 protein levels following YIPF4 knockdown.Results Both bioinformatic and IHC analyses demonstrated that YIPF4 and IER3IP1 were significantly upregulated in HCC tissues compared with adjacent non-tumor liver tissues(all P<0.05),and their expression levels were positively correlated(P<0.01).High YIPF4 and IER3IP1 expression showed no significant association with patient age,sex,HBV infection status,presence of cirrhosis,or serum AFP levels(all P>0.05),but correlated significantly with larger tumor size and ad-vanced TNM stage(all P<0.05).Functional assays revealed that YIPF4 knockdown markedly inhibited prolifera-tion,colony formation,migration,and invasion of HCC cells(all P<0.01).Western blot confirmed that IER3IP1 protein levels decreased following YIPF4 depletion.Conclusion YIPF4 is overexpressed in HCC and may drive ma-lignant progression by upregulating IER3IP1.Its expression correlates with aggressive clinicopathologic features and key malignant phenotypes in vitro,indicating that YIPF4-IER3IP1 axis could serve as a potential diagnostic and thera-peutic target in hepatocellular carcinoma.