蒙医温针对失眠大鼠脑干内GABA含量的影响OA

Objective To study the effect of Mongolian medicine warm acupuncture(MMWA)to stimulate Dinghui,Heyi and Xin acupoints on the expressions of γ-aminobutyric acid(GABA)positive cells in the brainstem.Methods The rats were ran-domly divided into blank control group,model group,MMWA group and traditional Chinese medicine acupuncture(TCMA)group,and the insomnia model was made by intraperitoneal injection of chlorophenylalanine(PCPA)for 2 consecutive days.In the MMWA group,Dinghui,Heyi and Xin acupoints were stimulated respectively.The TCMA group used acupuncture at Shenmen(HT7),Neiguan(PC6),Sanyinjiao(SP6)and Zusanli(ST36).The changes of general condition,fatigue tolerance,spleen index,thymus index and the number of GABA-positive cells in the brainstem of rats were viewed in each group.Results Compared with the model group,the MMWA group and the TCMA group showed statistically significant differences in weight gain(P＜0.05),and statistically significant differences in anti-fatigue ability,spleen index and thymus index(P＜0.01).The number of GABA-positive cells in the brainstem of rats in the mode group decreased to 9.24±2.65.After treatment,the number of GABA-positive cells in the brainstem of the MMWA group was 14.08±3.32,and that in the brainstem of the TCMA group was 13.79±3.15.The comparison of the two groups and the model group was statistically significant(P＜0.01),and the comparison between the MMWA group and the TCMA group was not statistically significant.Conclusion MMWA can increase the content of GABA in the brainstem of insomnia rats,and its sleep-promoting mechanism is closely related to its ability to regulate the content of GABA in the brainstem of insomnia rats.