超声联合血清PTPN3、PDK1检测对乳腺癌腋窝淋巴结转移的诊断价值OA
Diagnostic value of ultrasound combined with serum PTPN3,PDK1 detection for axillary lymph node metastasis in breast cancer
目的 探讨超声联合血清蛋白酪氨酸去磷酸酶3(PTPN3)、磷酸肌醇依赖性蛋白激酶-1(PDK1)检测对乳腺癌腋窝淋巴结转移的诊断价值.方法 选取2022年6月至2024年6月在湖南中医药大学第一附属医院进行乳腺癌手术治疗的136例患者作为研究对象.依据组织病理学检查结果,将发生乳腺癌腋窝淋巴结转移的患者归为转移组,未发生腋窝淋巴结转移的患者归为未转移组.所有患者均进行超声检查;采用酶联免疫吸附试验(ELISA)检测血清PTPN3、PDK1水平.绘制受试者工作特征(ROC)曲线分析血清PTPN3、PDK1对乳腺癌腋窝淋巴结转移的诊断价值;采用诊断试验四格表分析超声联合血清PTPN3、PDK1检测对乳腺癌腋窝淋巴结转移的诊断价值.结果 转移组76例,未转移组60例.与未转移组相比,转移组血清PTPN3、PDK1水平显著升高(P<0.05).转移组边界不清晰、淋巴结最大直径≥7 mm、纵横径比<2、内部回声不均匀、血流信号丰富占比均明显高于未转移组(P<0.05).超声联合血清PTPN3、PDK1检测诊断乳腺癌腋窝淋巴结转移的准确度为88.24%,灵敏度为96.05%,特异度为78.33%.3项联合诊断乳腺癌腋窝淋巴结转移的准确度、灵敏度明显高于各项单独检测(P<0.05).结论 乳腺癌腋窝淋巴结转移患者血清PTPN3、PDK1水平显著升高,超声联合血清PTPN3、PDK1检测对乳腺癌腋窝淋巴结转移的诊断效能更高,可为乳腺癌腋窝淋巴结转移的临床诊断提供参考.
Objective To explore the diagnostic value of ultrasound combined with serum protein tyrosine phosphatase non receptor type 3(PTPN3)and phosphoinositide dependent protein kinase 1(PDK1)detection for axillary lymph node metastasis in breast cancer.Methods A total of 136 patients who underwent breast cancer surgery from June 2022 to June 2024 in the First Affiliated Hospital of Hunan University of Chinese Medicine were selected as the research subjects.Based on the results of histopathological tests,patients with axillary lymph node metastasis of breast cancer were classified as the metastasis group,and those without axil-lary lymph node metastasis of breast cancer were classified as the non-metastasis group.Ultrasonography was performed on all patients.Enzyme-linked immunosorbent assay(ELISA)method was applied to measure the expression levels of serum PTPN3 and PDK1.The receiver operating characteristic(ROC)curve was drawn to analyze the diagnostic value of serum PTPN3 and PDK1 for axillary lymph node metastasis of breast cancer.The diagnostic value of serum PTPN3,PDK1 combined with ultrasound for axillary lymph node metastasis of breast cancer was analyzed by diagnostic test fourfold table.Results There were 76 cases in the metastasis group and 60 cases in the non-metastasis group.Compared with the non-metastasis group,serum PTPN3 and PDK1 levels were significantly elevated in the metastasis group(P<0.05),while the proportions of unclear borders,lymph node maximum diameter≥7 mm,length-to-width ratio<2,internal echo heterogeneity and a-bundant blood flow signals were obviously higher in the metastasis group(P<0.05).The accuracy,sensitivi-ty and specificity of ultrasound combined with serum PTPN3 and PDK1 in diagnosing axillary lymph node metastasis of breast cancer were 88.24%,96.05%and 78.33%respectively.Compared with the individual de-tection of each indicator,the accuracy,negative predictive value and sensitivity of the three-item combined di-agnosis for axillary lymph node metastasis of breast cancer are significantly higher(P<0.05).Conclusion The levels of serum PTPN3 and PDK1 in patients with axillary lymph node metastasis of breast cancer are ob-viously increased.Ultrasound combined with serum PTPN3 and PDK1 exhibits enhanced diagnostic accuracy in identifying metastatic axillary lymph nodes in breast cancer patients,which can provide reference for the clinical diagnosis of axillary lymph node metastasis in breast cancer.
汤欢;李林璐;罗佳;高怡江
湖南中医药大学第一附属医院超声影像科,湖南 长沙 410007湖南中医药大学第一附属医院超声影像科,湖南 长沙 410007湖南中医药大学第一附属医院超声影像科,湖南 长沙 410007湖南省人民医院输血科/血液病实验室,湖南 长沙 410005
医药卫生
蛋白酪氨酸去磷酸酶3磷酸肌醇依赖性蛋白激酶-1超声乳腺癌腋窝淋巴结转移
protein tyrosine phosphatase non receptor type 3phosphoinositide dependent protein kinase 1ultrasoundbreast canceraxillary lymph node metastasis
《检验医学与临床》 2026 (2)
171-176,6
湖南省教育厅科学研究项目(24B0348).
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