基于线粒体功能障碍探讨菌群代谢产物对艾滋病免疫功能重建的影响OA

objective To explore the effect of microbial metabolites on immune function reconstruction of AIDS based on mitochondrial dysfunction.Methods From October 2023 to March 2024,42 HIV-infected people who received antiretroviral therapy(ART)for 4 years were recruited,including 20 immune non-responders(INR)and 22 responders(IR).At the same time,20 non-infected MSM matched by sex and age were selected as the control group,and their HIV,hepatitis B or hepatitis C virus infection were all negative.The immune parameters were analyzed by flow cytometry.The levels of free fatty acid(FFA)and ferrous(Fe2+)in plasma were determined by fluorescence assay kit and iron colorimetric assay kit.Results The percentages of CD4+T cells,CD4+CD45RA+T cells and CD4+CD45RA+CD31+T cells in INR group were lower than those in control group and IR group(P<0.05).Compared with IR group,the JC-1 ratio of CD4+T cells,the level of reactive oxygen species(ROS)and the peroxidation/antioxidation of C11-BODIPY in INR group increased(P<0.05).The lipid ROS level of CD4+T cells in IR group was higher than that in control group(P<0.05).The FFA level and Fe2+level in peripheral blood of patients in INR group were higher than those in control group and IR group(P<0.05),and the percentage of CD4+CD36+T cells was lower than that in control group and IR group(P<0.05),but there was no significant difference between control group and IR group.Conclusion The increase of FFA,a metabolite of flora,is accom-panied by the decrease of CD36 receptor expression on CD4+T cells,leading to mitochondrial dysfunction(P>0.05).