NANOG表达对食管鳞状细胞癌进展的促进作用OA

Objective To investigate the expression characteristics of NANOG in esophageal squamous cell car-cinoma(ESCC)tissues and its influence on the proliferation and migration of ESCC cells.Methods Immunohisto-chemistry was used to detect the expression of NANOG and Survivin in 107 cases of ESCC tissues and adjacent esopha-geal mucosal tissues and their correlations with clinicopathological features as well as patient prognosis were analyzed.A NANOG-overexpressing ESCC cell model(KYSE30)was constructed using lentiviral transfection.Cell prolifera-tion,migration,cell cycle distribution and apoptosis in KYSE30 cells were assessed using CCK-8,colony formation,scratch assay,Transwell chamber assay and flow cytometry.Western blot was performed to detect the effects of NANOG overexpression on the expression of E-cadherin,vimentin,Survivin,VEGF and Cyclin D1.Results NANOG cytoplasmic positivity was observed in 48.6%of ESCC cases,which was significantly negatively correlated with progression-free survival(P=0.001),but showed no significant association with overall survival(P=0.077).NANOG nuclear positivity was detected in 75.7%of ESCC cases,which was positively correlated with lymph node staging(P=0.003),but was not associated with overall survival or progression-free survival(P=0.961,P=0.972).Functional assays demonstrated that NANOG overexpression significantly promoted KYSE30 cell prolifera-tion and migration,increased the S-phase cell population,and suppressed apoptosis.Concurrently,it downregulated E-cadherin while upregulating the expression of vimentin,Survivin,VEGF,and Cyclin D1.Conclusion The cyto-plasmic positive expression of NANOG may serve as a biomarker for tumor progression in ESCC and is expected to be-come a potential therapeutic target for ESCC in the future.