AKR1C3通过PD1/PD-L1信号通路对乳腺癌细胞恶性生物学行为的干预作用OA

Objective To investigate the effect of aldo-keto reductase family 1 member C3(AKR1C3)on malignant biological behavior of breast cancer cells and its influence on the programmed cell death protein 1/programmed death-ligand 1(PD-1/PD-L1)pathway.Methods MCF-7 human breast cancer cells with NC and AKR1C3 groups transfected with NC plasmid and AKR1C3 plasmid respectively.Cell viability at 24 h/48 h/72 h post-transfection was assessed by MTT assay;flow cytometry measured cell survival rate and early/late apoptosis ratios;Transwell evaluated migration and invasion capabilities;western blot detected PD-1,PD-L1,protein kinase B(AKT)protein expression.For in vivo experiments,the C57BL/6 mice were used to establish tumor-bearing models.Human breast cancer MCF-7 cells transfected with NC plasmid and AKR1C3 plasmid were used for cell tumor-bearing.The tumor volume was measured every 3 days for 21 days,draw the tumor growth curves of the two groups of mice and measure the tumor mass at the end of the experiment.Results Compared to NC groups,the AKR1C3 group showed significantly reduced cell viability(time-dependent)(P<0.05),suppressed m igration/invasion(P<0.05),increased early/late apoptosis ratios(P<0.05),and downregulated PD-1/PD-L1/AKT protein expression(P<0.05).In vivo,AKR1C3 group exhibited reduced tumor volume and weight(P<0.05).Conclusion AKR1C3 inhibits malignant biological behaviors in breast cancer cells and suppresses PD-1/PD-L1 signaling pathway protein expression.