miR-222对奶牛乳腺上皮细胞乳脂代谢的影响OA

The function of miR-222 in bovine mammary epithelial cells(bMECs),as a miRNAs dif-ferentially expressed in high-fat and low-fat Chinese Holstein cows,is still unclear.Therefore,this study aims to verify the effects of miR-222 on milk fat metabolism in cow mammary epithelial cells,and improve the molecular mechanism of miRNA regulating milk fat synthesis in cows.The overex-pression and interference vectors of miR-222 were transfected into bMECs respectively to detect the contents of triglycerides and cholesterol in bMECs.At the same time,the proliferation and apoptosis of bMECs were detected.The results showed that overexpression of miR-222 could significantly re-duce the contents of triglycerides in cells(P<0.05)and intracellular cholesterol(P<0.05)compared with miR-shNC group.It significantly promoted the mRNA expression of PPARA,ELOVL6,ACSL1,and FADS1 genes involved in fatty acid metabolism.It can significantly inhibit the growth of mam-mary epithelial cells and promote their apoptosis.The results showed that miR-222 was not only in-volved in regulating the fate of bMECs,but also involved in regulating the milk fat synthesis and fatty acid metabolism of bMECs,laying a foundation for further exploring the regulatory role and molecu-lar mechanism of miR-222 in the milk fat metabolism of bMECs significantly correlated with lactose status(P<0.05).The results of this study can provide a basis for molecular breeding of Chinese Hol-stein dairy cows and further improving the quality and performance of dairy products.