菜豆原生质体的融合条件研究OA

Protoplast fusion is an effective means to create and breed new varieties of strictly self-pollinated crops.PEG-high Ca2+-high pH fusion method is an ideal method to promote protoplast fu-sion with high efficiency.To establish a protoplast fusion system for common bean,the protoplast fu-sion of common bean was induced by PEG-high Ca2+-high pH method,and the suitable fusion meth-ods and conditions for bean protoplasts were studied.The results showed that the optimal conditions for protoplast fusion in beans are as follows:a protoplast density of 1.3×105/mL,initial induction of fusion with 35%PEG(MW=6 000),followed by the sequential addition of 20 µL of high Ca2+-high pH solution four times,incubation at room temperature for 60-90 min,and finally washing to pro-mote fusion with with 80 µL of high Ca2+-high pH solution for 2-3 times,whcih can achieve a fusion rate of up to 13%.