2株基因2型鹅星状病毒的分离鉴定及遗传进化分析OA

Goose astrovirus genotype 2(GAstV-2)is considered as the major agent of fatal visceral gout in goslings.In this study,based on the GAstV-2 isolated in Shandong Province previously,2 strains of GAstV-2 were further isolated during specific period in 2023.Both viral full-length genomes were sequenced and genetic evolutionary analysis was conducted.Our results indicated that the strains isolated in this study were both GAstV-2 subgenotype Ⅱ-c,indicating that subgenotypeⅡ-c was still the dominant genotype in China.Phylogenetic analysis of genome and three open reading frames demonstrated that G666 and G667 were clustered with G529,G538,G548 and G576,which were isolated in Shandong.Moreover,several regular and characteristic mutations were observed in the six strains.The mutations contained three novel genetic markers(positions 483 in ORF1b,and position 228 and 376 in ORF2)and six"temperature response"residues suggested in previous study,demonstrating viral durative evolution.Overall,these findings improved our understanding for epidemiology and genetic evolution of GAstV-2 and contributed to the development of vaccines and diagnostics.