高效液相色谱-质谱法分析甘蔗梢多酚类化合物OA

To establish an efficient detection method for polyphenolic compounds in sugarcane tops,this study employed high performance liquid chromatography-mass spectrometry(HPLC-MS)for systematic qualitative and quantitative analysis of polyphenols in sugarcane tops.The samples were extracted via composite enzyme-assisted extraction(1.6%composite enzyme,pH 5.0,56℃,30 min),purified by macroporous resin column chromatography,and finally filtered through a 0.22 μm microporous membrane before injection.The results showed that eight polyphenol monomers were identified in the sugarcane top extract,including quinic acid,benzoic acid,caffeic acid,apigenin,p-coumaric acid,chlorogenic acid,ferulic acid,and gallic acid.Among these,p-coumaric acid,ferulic acid,chlorogenic acid,and gallic acid were commonly present in the samples,with concentrations ranging from 1.8 to 30 mg/g.Further methodological validation for the quantification of these four components demonstrated good linearity(correlation coefficient R2＞0.99),spike recovery rates between 90.78%and 101.06%,relative standard deviation(RSD)for precision tests ranging from 0.26%to 2.24%(n=6),method detection limits of 0.1～0.4 mg/kg,and quantification limits of 0.5～2.1 mg/kg.This method exhibits excellent separation efficiency,high sensitivity,and good reproducibility,making it suitable for the rapid qualitative and quantitative analysis of polyphenols in sugarcane tops.It provides a reliable analytical foundation for further development and utilization of sugarcane tops in functional foods and pharmaceuticals.