紫朱软膏治疗糖尿病足溃疡的临床疗效及对创面中性粒细胞胞外陷阱和NLRP3、IL-1表达的影响OA
Clinical efficacy of Zizhu ointment on diabetic foot ulcer and its effect on neutrophil extracellular trap and expression of NLRP3 and IL-1 in the wounds
目的:评估紫朱软膏对糖尿病足溃疡患者的临床疗效及其对患者创面中性粒细胞胞外陷阱(NETs)及炎症因子NOD样受体基因家族P3(NLRP3)、白细胞介素1(IL-1)表达的影响.方法:选取2024年1～6月在上海中医药大学附属曙光医院接受治疗的92例糖尿病足溃疡患者,在采取基础治疗后,随机分为试验组和对照组,每组46例,试验组采用紫朱软膏外敷,对照组采用外喷贝复济(bFGF)并用凡士林纱布外敷治疗.治疗8周后,比较两组的溃疡愈合率、溃疡面积和溃疡深度.采集两组患者治疗后第3天和第7天脱落的溃疡组织标本,采用苏木素-伊红(H&E)染色、免疫荧光染色和免疫组化观察创面炎性细胞数、NETs数、NLRP3和IL-1的表达.结果:与本组治疗前相比,两组患者的溃疡面积和溃疡深度均明显降低(P<0.01).治疗后试验组的溃疡愈合率明显高于对照组(P<0.01),试验组的溃疡面积、溃疡深度明显低于对照组(P<0.01).与正常皮肤组织相比,两组患者创面的炎性细胞数、NETs数及NLRP3、IL-1表达均明显升高(P<0.01).与治疗前相比,治疗后两组患者的创面炎性细胞数、NETs数及NLRP3、IL-1表达均明显降低(P<0.01).与对照组相比,试验组在治疗3 d和7 d后创面炎性细胞数、NETs数、NLRP3和IL-1表达明显降低(P<0.01).结论:紫朱软膏外用具有明显的临床疗效,能够促进糖尿病足溃疡愈合.其机制可能涉及抑制NETs形成以及炎症因子NLRP3和IL-1的表达.
Objective:To evaluate the clinical efficacy of Zizhu Ointment in the treatment of diabetic foot ulcer and its effects on neutrophil extracellular traps(NETs)and the expression of inflammatory factors NLRP3 and IL-1 in wound tissues.Methods:A total of 92 diabetic foot ulcer patients treated at Shuguang Hospital affiliated with Shanghai University of Traditional Chinese Medicine from January to June 2024 were randomly divided into an experimental group and a control group,each consisting of 46 cases,after receiving basic treatment.The experimental group applied Zizhu Ointment topically,while the control group applied basic fibroblast growth factor(bFGF)spray and covered with Vaseline gauze for treatment.After 8 weeks of treatment,the ulcer healing rate,ulcer area and ulcer depth of both groups were compared.Specimens of ulcer tissue shed on the 3rd and 7th day after treatment from both groups were collected and observed for the number of inflammatory cells,NETs,and the expression of NL-RP3 and IL-1 in the wound using Hematoxylin-Eosin(H&E)staining,immunofluorescence staining,and immunohistochemistry.Results:Compared to before treatment in the same group,the ulcer area and ulcer depth in both groups were significantly reduced(P<0.01).After treatment,the ulcer healing rate in the experimental group was significantly higher than that in the control group(P<0.01),and the ulcer area,ulcer depth in the experimental group were significantly lower than those in the control group(P<0.01).Compared to normal skin tissue,the number of inflammatory cells,NETs,and the expression of NLRP3 and IL-1 in the wound tissues of both groups were significantly increased(P<0.01).Compared to before treatment,the number of inflammatory cells,NETs,NLRP3 and IL-1 expression in the wound of the two groups were significantly decreased after treatment(P<0.01).Compared to the control group,the number of inflammatory cells,NETs,and the expression of NLRP3 and IL-1 in the wound tissues of the experimental group were significantly reduced after 3 and 7 days of treatment(P<0.01).Conclusion:The external use of Zizhu Ointment in the treatment of diabetic foot ulcer has significant clinical efficacy and can promote the healing of diabetic foot ulcers.Its mechanism may involve the inhibition of NETs formation and the expression of inflammatory factors NLRP3 and IL-1.
孙健;樊炜静;袁美杰;黄何尘;游洋;柳国斌
上海中医药大学附属曙光医院,上海 201203上海中医药大学附属曙光医院,上海 201203上海中医药大学附属曙光医院,上海 201203上海中医药大学附属曙光医院,上海 201203上海中医药大学附属曙光医院,上海 201203上海中医药大学附属曙光医院,上海 201203
医药卫生
糖尿病足溃疡紫朱软膏临床疗效中性粒细胞胞外诱捕网NOD样受体基因家族P3(NLRP3)白细胞介素1(IL-1)
Diabetic foot ulcerZizhu ointmentClinical efficacyNeutrophil extracellar trapsNOD-like receptor family pyrin domain containing 3(NLRP3)Interleukin-1(IL-1)
《海南医科大学学报》 2026 (1)
1-9,9
This study was supported by the National Natural Science Foundation of China(82274528)Pudong New Area Health Committee Health and Family Planning Research Project(PW2020E-4) 国家自然科学基金项目(82274528)浦东新区卫生健康委员会科研项目(PW2020E-4)
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