秸秆还田配施复合微生物菌剂对退化褐土修复及关键微生物筛选OA

[Objective]To investigate the effects of straw returning combined with microbial agent on soil nutrients and microbial community diversity in degraded cinnamon soil,and to provide effective and feasible restoration measures for soils degraded.[Method]Using untreated degraded cinnamon soil as the control(CK),straw alone(T1)and a combination of straw and microbial agent(Bacillus stutilis and Metarhizium)(T2)were applied.Soil nutrient indicators were measured,and metage-nomic sequencing was used to analyze differences in soil microbial communities across treatments.Key microbial taxa associated with soil organic carbon(SOC)were identified.[Result]The addition of straw and microbial agent improved soil pH and increased the content of SOC,available potassium(AK),available phosphorus(AP),alkali-hydrolyzable nitrogen(AN),and microbial biomass carbon(MBC).Metagenomic sequencing results indicated that the combined application of straw and micro-bial agent enhanced the α diversity of soil microorganisms at the species level.The relative abundance of Pseudomonadota,Actinomycetota,and Bacteroidota in the soil significantly increased.Correlation analysis revealed that SOC and microbial biomass carbon were closely related to microbial community structure.Among the microbial taxa with significant differences,Nonomuraea(Actinobacteria)was significantly correlated with changes in organic carbon and may play a key role in the restoration of degraded cinnamon soil.[Conclusion]In the process of restoring degraded cinnamon soil,the com-bined application of straw and microbial agent can significantly improve soil nutrient levels,alter the composition of soil microbial species,and enhance α diversity at the species level,and screen Nono-muraea as the keystone microbial taxa.