猪circTEX9生物学特性及其对猪肌内前体脂肪细胞的影响OA

[Objective]The biological characteristics of porcine circTEX9 were identified and the regu-lation role of porcine circTEX9 on intramuscular fat deposition was studied.[Method]Based on the previous sequencing data of our research group,circTEX9 was selected as the research object,and its ring structure,stability and subcellular localization were comprehensively identified and analyzed by Sanger sequencing and subcellular localization.At the same time,the regulatory effects of circTEX9 on the proliferation and differentiation of porcine intramuscular preadipocytes were investigated by overexpression method,and the downstream target genes regulated by circTEX9 and the signal path-ways involved were predicted by combining biological information methods.[Result]Porcine circ-TEX9 is a circular RNA molecule composed of exons 7 to 10 of TEX9 gene,which is resistant to RNase R and mainly exists in the cytoplasm.circTEX9 has a stable secondary structure and contains two open reading frames(ORF)and two internal ribosome entry sites(IRES)with translation poten-tial.circTEX9 significantly inhibited the expression levels of proliferative marker genes,and signifi-cantly decreased cell viability.The expression levels of differentiation marker genes were significantly upregulated.Based on the above results and bioinformatics analysis,it was predicted that circTEX9 could absorb ssc-miR-204,ssc-miR-23a and ssc-miR-23b through competing endogenous RNA(ceRNA)network to regulate downstream target genes.These target genes exert biological functions by participating in the adenosine 5 ′-monophosphate-activated protein kinase signaling pathway,cal-cium signaling pathway and mitogen-activated protein kinase signaling pathway,ultimately influencing porcine intramuscular preadipocytes.[Conclusion]circTEX9 has high stability in pig vivo and inhibits the proliferation of intramuscular preadipocytes while promoting lipid deposition,potentially exerting its biological function through ceRNA network.