lncRNA XIST调控miR-17-5p/FOSL1轴对胆囊癌细胞增殖、迁移和侵袭的影响OA

Objective To investigate the effects of long non coding RNA(lncRNA)X chromosome inactive specific transcript(XIST)on the proliferation,migration,and invasion of gallbladder cancer(GBC)cells by adjusting the microRNA-17-5p(miR-17-5p)/FOS like antigen 1(FOSL1)axis.Methods qRT-PCR was used to detect mRNA expression in GBC tissues and cell lines.The dual luciferase reporter gene assay was used to validate the interaction between lncRNA XIST and miR-17-5p,as well as miR-17-5p and FOSL1.EH-GB1 cells were separated into Ctrl group,sh-NC group,sh-XIST group,sh-XIST+inhibitor-NC group,sh-XIST+inhibitor-miR-17-5p group,mimic-NC group,mimic-miR-17-5p group,mimic-miR-17-5p+OE-NC group,and mimic-miR-17-5p+OE-FOSL1 group.Trypan blue staining and plate cloning experiments were performed to detect EH-GB1 cell proliferation.The scratch test was performed to measure EH-GB1 cell migration.Transwell experiment was performed to detect EH-GB1 cell invasion.Western blotting was used to measure the expression of Ki67,Cyclin D1,MMP-2,CD44 and FOSL1 proteins in EH-GB1 cells.The nude mouse transplant tumor experiment was used to measure the effect of knocking down lncRNA XIST on the growth of GBC tumors.Results miR-17-5p was lowly expressed in GBC tissues and cells,while lncRNA XIST and FOSL1 were highly expressed.The sh-XIST group had lower survival rate,proliferation rate,scratch healing rate,invasion rate,Ki67,Cyclin D1,MMP-2,and CD44 levels than the sh-NC and Ctrl groups(P<0.05).The sh-XIST+inhibitor-miR-17-5p group had higher survival rate,proliferation rate,scratch healing rate,invasion rate,Ki67,Cyclin D1,MMP-2,and CD44 levels than the sh-XIST group and sh-XIST+inhibitor-NC group(P<0.05).The mimic-miR-17-5p group had lower survival rate,proliferation rate,scratch healing rate,invasion rate,Ki67,Cyclin D1,MMP-2,CD44 and FOSL1 levels than the mimic-NC group and Ctrl group(P<0.05).The mimic-miR-17-5p+OE-FOSL1 group had higher survival rate,proliferation rate,scratch healing rate,invasion rate,Ki67,Cyclin D1,MMP-2,CD44 and FOSL1 levels than the mimic-miR-17-5p group and the mimic-miR-17-5p+OE-NC group(P<0.05).lncRNA XIST could target and negatively regulate miR-17-5p,and miR-17-5p could target and negatively regulate FOSL1(P<0.05).The XIST knockdown group had lower tumor volume,weight,FOSL1 protein,and lncRNA,while higher miR-17-5p than the negative control group(P<0.05).Conclusion lncRNA XIST promotes the proliferation,migration,and invasion of GBC cells by adjusting the miR-17-5p/FOXL1 axis.