LHPP过表达对胰腺癌的抑制作用OA

Objective To investigate the inhibitory effect of phospholysine phosphohistidine inorganic pyrophosphate phosphatase(LHPP)over-expression on pancreatic cancer.Methods Cancer tissues and paired adjacent tissues from 18 pancreatic cancer patients admitted to Inner Mongolia People's Hospital between June 2022 and June 2025 were collected retrospectively,and LHPP mRNA expression was detected via RT-qPCR.Human pancreatic cancer cell lines Capan-2 and Panc-1,along with the normal pancreatic cell line HPDE6-C7,were selected to examine LHPP mRNA and protein expression using RT-qPCR and Western blotting.The LHPP over-expressed plasmid was constructed and extracted,then transfected into Capan-2 and Panc-1 cells using the liposome method to establish LHPP over-expressed cell lines(over-expressed group),with empty vector-transfected cells serving as controls(empty vector group).Cell proliferation was detected by CCK-8 assay,invasion ability was evaluated using Transwell assay,migration was analyzed via wound healing assay,and apoptosis was measured by flow cytometry.Results In pancreatic cancer tissues,LHPP mRNA expression was significantly lower than in paired adjacent tissues(P<0.01).The mRNA and protein expression levels of LHPP in pancreatic cancer cell lines Capan-2 and Panc-1 were significantly lower than in the normal pancreatic cell line HPDE6-C7(P<0.05).After transfection with the LHPP over-expressed plasmid,both mRNA and protein levels of LHPP were significantly upregulated in Capan-2 and Panc-1 cells.CCK-8 assay showed that on day 3,4,and 5 after transient transfection with LHPP,the relative cell viability in the over-expressed groups was significantly lower than that in the empty vector group(P<0.01).Transwell assay results indicated that the number of invading cells in the LHPP over-expressed group was significantly reduced compared with the empty vector group(P<0.001).Wound healing assay demonstrated that cell migration was significantly inhibited in the LHPP over-expressed group(P<0.05).Flow cytometry analysis revealed that the apoptosis rate was significantly higher in the LHPP over-expressed Capan-2 and Panc-1 cells than that in the empty vector control group(P<0.05).Conclusion Over-expression of LHPP can significantly inhibit the proliferation,migration,and invasion of pancreatic cancer cells and promote their apoptosis.